DNA-mediated delivery of lipophilic molecules via hybridization to DNA-based vesicular aggregates
DNA-mediated delivery of lipophilic molecules via hybridization to DNA-based vesicular aggregates
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DOI：
10.1021/la053005o
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发表时间：
2006-03-28
期刊：
影响因子：
3.9
通讯作者：
Sprague, M
中科院分区：
文献类型：
--
作者：
Dentinger, PM;Simmons, BA;Sprague, M
提出了一种通过DNA杂交稳定疏水性分子并将其释放到水溶液中的方案。十四烷基疏水尾共价连接到合成低聚物。并且所得的两亲分子在水性环境中吸收Lip大量的橙子OT和芘染料。所得结构不影响表面张力，并且如光散射和TEM所示主要是球形的，并且芘荧光与疏水环境一致。它的结论是，两亲性的DNA创建囊泡域上的疏水染料驻留，并在溶液中稳定。在暴露于互补链时，芘染料从结构中释放。表明该方案可用于未标记或DNA介导的药物递送。
A scheme is presented for stabilizing hydrophobic molecules and releasing them into aqueous solution via DNA hybridization. A tetradecyl hydrophobic tail is covalently attached to synthetic oligomers. and the resulting amphiphilic molecules take Lip substantial amounts of orange OT and pyrene dyes in aqueous environments. The resulting structures do not affect the surface tension and are predominantly spherical as shown by light scattering and TEM, and the pyrene fluorescence is consistent with a hydrophobic environment. It is Concluded that the amphiphilic DNA creates vesicular domains upon which the hydrophobic dyes reside and are stabilized in solution. Upon exposure to the complementary strand, the pyrene dye is released from the structures. showing that the scheme can be used for unlabeled or DNA-mediated drug delivery.