The novel preventive effect of Daikenchuto (TJ-100), a Japanese herbal drug, against neonatal necrotizing enterocolitis in rats
The novel preventive effect of Daikenchuto (TJ-100), a Japanese herbal drug, against neonatal necrotizing enterocolitis in rats
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日本草药大研中汤（TJ-100）对大鼠新生坏死性小肠结肠炎的新预防作用
DOI：
10.1007/s00383-017-4145-9
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发表时间：
2017
影响因子：
1.8
通讯作者：
Ieiri Satoshi
中科院分区：
文献类型：
--
作者：
Shinyama Shin;Kaji Tatsuru;Mukai Motoi;Nakame Kazuhiko;Matsufuji Hiroshi;Takamatsu Hideo;Ieiri Satoshi
坏死性小肠结肠炎(NEC)是一种严重危害早产儿健康的胃肠道疾病。[方法]采用新生大鼠窒息模型(100%N290次，每4小时1次)+内毒素(4 mg/kg/d，第0、1天口服)诱导新生大鼠NEC。分4组(对照组：0 mg/kg/d，I组：0.3 g/kg/d，II组：0.6 g/kg/d，III组：1.0 g/kg/d)进行疗效评价。通过微导管将Daikichuto注射到胃内。根据Dovorak的先前报告，使用NEC分级对NEC的发病率和严重程度进行了病理评估。结果对照组、I组、II组、III组NEC发生率分别为68.7%、30.0%、30.7%、13.3%。大剂量组大鼠NEC发生率明显升高，IL-6阳性细胞率明显低于对照组(P=0.04)。结论大剂量大剂量组可通过减少IL-6的产生来降低NEC的发生率。
PurposeNecrotizing enterocolitis (NEC) is a devastating gastrointestinal disease of premature infants. Daikenchuto, a Japanese herbal drug, has several effects on the digestive system, so we investigated its preventive effects in a rat model of NEC.MethodsNEC was induced in newborn rats via asphyxia (100% N2for 90 s; every 4 h) + LPS (4 mg/kg/day [administered orally on days 0 and 1]). The effects of Daikenchuto were evaluated in four groups (control: 0 g/kg/day, I: 0.3 g/kg/day, II: 0.6 g/kg/day, and III: 1.0 g/kg/day). Daikenchuto was administered into the stomach through a microcatheter. The incidence and severity of NEC were pathologically assessed using the NEC grade in accordance with Dovorak’s previous report. Cell positivity for inflammatory cytokine (IL-6) was also evaluated.ResultsDaikenchuto reduced the incidence of NEC in control, Groups I, II, and III to 68.7, 30.0, 30.7, and 13.3%, respectively. High-dose Daikenchuto significantly improved the incidence of NEC, and the rate of IL-6 positive cells in group III was significantly lower than in the control group (p= 0.04).ConclusionWe evaluated the effect of Daikenchuto against NEC and found that it reduced the incidence rate of NEC due to a decrease in the IL-6 production.