Association between Wisteria floribunda agglutinin-positive Mac-2 binding protein and the fibrosis stage of non-alcoholic fatty liver disease
Association between Wisteria floribunda agglutinin-positive Mac-2 binding protein and the fibrosis stage of non-alcoholic fatty liver disease
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DOI：
10.1007/s00535-014-1007-2
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发表时间：
2015-07-01
影响因子：
6.3
通讯作者：
Narimatsu, Hisashi
中科院分区：
文献类型：
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作者：
Abe, Masanori;Miyake, Teruki;Narimatsu, Hisashi
准确评估非酒精性脂肪性肝病（NAFLD）患者的肝纤维化对于识别可能发生并发症的患者非常重要。本研究的目的是（1）使用基于聚糖糖链的免疫测定法测定血清花紫藤凝集素阳性Mac-2结合蛋白（WFA（+）-M2 BP），（2）将结果与纤维化的临床评估进行比较。组织学检查结果由3位经验丰富的肝脏病理学家进行评估。对于0期（n = 35）、1期（n = 113）、2期（n = 49）、3期（n = 41）和4期（n = 51）肝纤维化，血清WFA（+）-M2 BP临界指数分别为0.57、0.70、1.02、1.57和2.96。多元回归分析显示血清WFA（+）-M2 BP值与纤维化分期（2期）相关。血清WFA（+）-M2 BP的受试者工作特征曲线下面积（AUROC）、敏感性和特异性在严重纤维化（3期的千分之一）中分别为0.876、85.9和74.6%，在肝硬化中分别为0.879、74.6和87.0%。血清WFA（+）-M2 BP与6种非侵袭性常规指标相比，在诊断严重肝纤维化和肝硬化方面具有最大的AUROC，血清WFA（+）-M2 BP值可用于评估NAFLD患者的肝纤维化分期。
Accurately evaluating liver fibrosis in patients with non-alcoholic fatty liver disease (NAFLD) is important for identifying those who may develop complications. The aims of this study were (1) to measure serum Wisteria floribunda agglutinin-positive Mac-2 binding protein (WFA(+)-M2BP) using the glycan sugar chain-based immunoassay and (2) to compare the results with clinical assessments of fibrosis.Serum WFA(+)-M2BP values were retrospectively evaluated in 289 patients with NAFLD who had undergone liver biopsy. Histological findings were evaluated by three blinded, experienced liver-specific pathologists.For stages 0 (n = 35), 1 (n = 113), 2 (n = 49), 3 (n = 41), and 4 (n = 51) of liver fibrosis, the serum WFA(+)-M2BP cutoff indexes were 0.57, 0.70, 1.02, 1.57, and 2.96, respectively. Multivariate regression analysis showed that serum WFA(+)-M2BP values were associated with the stage of fibrosis (a parts per thousand yenstage 2). The areas under the receiver operating characteristic curve (AUROC), sensitivity, and specificity of serum WFA(+)-M2BP were 0.876, 85.9, and 74.6 %, respectively, for severe fibrosis (a parts per thousand yenstage 3) and were 0.879, 74.6, and 87.0 %, respectively, for cirrhosis. When compared with six non-invasive conventional markers, serum WFA(+)-M2BP had the greatest AUROC for diagnosing severe fibrosis and cirrhosis.Serum WFA(+)-M2BP values are useful for assessing the stage of liver fibrosis in patients with NAFLD.