Deciphering links between bacterial interactions and spatial organization in multispecies biofilms
Deciphering links between bacterial interactions and spatial organization in multispecies biofilms
复制标题
DOI：
10.1038/s41396-019-0494-9
复制
发表时间：
2019-12-01
期刊：
影响因子：
11
通讯作者：
Sorensen, Soren J.
中科院分区：
文献类型：
--
作者：
Liu, Wenzheng;Jacquiod, Samuel;Sorensen, Soren J.
环境微生物经常生活在多物种生物膜中，其中可能发生互利关系和共同进化，定义了成员物种和整体社区功能的空间组织。在这种情况下，从微生物的相互作用，如有效的组织优化生长和活动的多物种生物膜，可能成为适应性选择的对象出现的内在属性。然而，鲜为人知的是，在建立一个可预测的空间组织内的多物种生物膜的基本种间相互作用的性质，我们提出了一个比较metatranscriptomic分析的细菌菌株居住在三个物种和四个物种的生物膜，旨在破译的分子机制，支持细菌的相互作用负责显着增强的生物量生产和相关的典型的空间组织，他们显示。Metatranscriptomic配置文件同意在微网站占领的变化，以响应添加/删除一个单一的物种，被驱动的合作，竞争和促进过程。我们的结论是，四种生物膜的生物量生产的增强是一种内在的社区财产出现微调空间优化实现通过协调一致的拮抗和互利互惠的相互作用，其中每个物种占据一个定义的微型网站有利于自己的增长。我们的研究结果进一步说明了如何分子机制可以更好地解释时，实际的微观空间组织的视觉成像的支持下，我们提出的表型适应选择社会相互作用的分子机制稳定微生物群落。
Environmental microbes frequently live in multispecies biofilms where mutualistic relationships and co-evolution may occur, defining spatial organization for member species and overall community functions. In this context, intrinsic properties emerging from microbial interactions, such as efficient organization optimizing growth and activities in multispecies biofilms, may become the object of fitness selection. However, little is known on the nature of underlying interspecies interactions during establishment of a predictable spatial organization within multispecies biofilms We present a comparative metatranscriptomic analysis of bacterial strains residing in triple-species and four-species biofilms, aiming at deciphering molecular mechanisms underpinning bacterial interactions responsible of the remarkably enhanced biomass production and associated typical spatial organization they display. Metatranscriptomic profiles concurred with changes in micro-site occupation in response to the addition/removal of a single species, being driven by both cooperation, competition, and facilitation processes. We conclude that the enhanced biomass production of the four-species biofilm is an intrinsic community property emerging from finely tuned space optimization achieved through concerted antagonistic and mutualistic interactions, where each species occupies a defined micro-site favoring its own growth. Our results further illustrate how molecular mechanisms can be better interpreted when supported by visual imaging of actual microscopic spatial organization, and we propose phenotypic adaptation selected by social interactions as molecular mechanisms stabilizing microbial communities.