Molecular characterization of dopamine receptors.
Molecular characterization of dopamine receptors.
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多巴胺受体的分子表征。
DOI：
10.1093/ajh/3.6.29s
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发表时间：
1990
影响因子：
3.2
通讯作者：
Caron,MG
中科院分区：
文献类型：
--
作者：
Bates,MD;Gingrich,JA;Bunzow,JR;Falardeau,P;Dearry,A;Senogles,SE;Civelli,O;Caron,MG
d1和d2多巴胺受体已分别使用基于亚型选择性拮抗剂SCH 23390和spiperone的特异性探针进行生化表征。通过光亲和标记法从多个组织中鉴定出d2多巴胺受体，并利用亲和、凝集素和羟基磷灰石层析相结合的方法从牛垂体前叶中纯化到均匀性。利用β2-肾上腺素能受体基因的一部分作为探针，通过低严格杂交技术克隆了一个编码大鼠脑多巴胺受体的互补DNA （cDNA）。光亲和交联和亲和层析也被用于鉴定和纯化大鼠脑d1多巴胺受体。Am J Hypertens 1990；3:29S-33S
The D1and D2dopamine receptors have been biochemically characterized using specific probes based on the subtype selective antagonists SCH 23390 and spiperone, respectively. The D2dopamine receptor was identified from several tissues by photoaffinity labeling and was purified from bovine anterior pituitary to homogeneity using a combination of affinity, lectin and hydroxylapatite chromatography. A complementary DNA (cDNA) encoding a rat brain D2dopamine receptor has been cloned via low stringency hybridization using a portion of theβ2-adrenergic receptor gene as a probe. Photoaffinity crosslinking and affinity chromatography have also been used to identify and purify the rat brain D1dopamine receptor. Am J Hypertens 1990;3:29S-33S