The characterization and localization of the mouse thymopoietin lamina-associated polypeptide 2 gene and its alternatively spliced products
The characterization and localization of the mouse thymopoietin lamina-associated polypeptide 2 gene and its alternatively spliced products
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DOI：
10.1101/gr.6.5.361
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发表时间：
1996-05-01
期刊：
影响因子：
7
通讯作者：
Simon, AJ
中科院分区：
文献类型：
--
作者：
Berger, R;Theodor, L;Simon, AJ
胸腺生成素（Thymopoietins，Tmpos）是一类广泛表达的核蛋白，与核纤层相关多肽2（lamina-associated polypeptide 2，LAP 2）具有同源性。在这里，我们报告了七个小鼠Tmpo mRNA转录本的分离和表征，命名为Tmpo α，β，β '，γ，δ，δ和ζ。α、β和γ Tmpo cDNA克隆分别是先前表征的人α、β和γ TMPO的小鼠同源物，而Tmpo β、δ和ζ是新的cDNA。此外，小鼠Tmpo基因的克隆和表征。TMPO基因位于小鼠10号染色体的中心区域，编码所有已知的TMPO cDNA序列。人类TMPO基因与小鼠TMPO基因的基因组结构几乎完全相同，并且在小鼠中发现了新的可变剪接转录本，这促使我们重新分析人类TMPO基因。发现人β特异性结构域由3个外显子编码，命名为6a、6 b和6c，而不是如前所述由单个外显子编码。这些发现表明，可能有更多的人类转录比目前公认的，可能参与的新增长的家庭TMPO蛋白在核结构和细胞周期控制进行了讨论。
Thymopoietins (Tmpos) are a group of ubiquitously expressed nuclear proteins, with sequence homology to lamina-associated polypeptide 2 (LAP2). Here we report the isolation and characterization of seven mouse Tmpo mRNA transcripts named Tmpo alpha, beta, beta', gamma, epsilon, delta, and zeta. The alpha, beta, and gamma Tmpo cDNA clones are the mouse homologs of the previously characterized human alpha, beta, and gamma TMPOs, respectively, whereas Tmpo epsilon, delta, and zeta are novel cDNAs. Additionally, the mouse Tmpo gene was cloned and characterized. It is a single-copy gene organized in 10 exons spanning similar to 22 kb, which encodes all of the described Tmpo cDNA sequences, located in the central region of mouse chromosome 10, The almost identical genomic organization between the human and mouse genes, and the novel alternatively spliced mouse transcripts, led us to reanalyze the human TMPO gene. The human beta-specific domain was found to be encoded by 3 exons designated 6a, 6b, and 6c and not by a single exon as described previously. These findings suggest that there may be more human transcripts than currently recognized, The possible involvement of the new growing family of Tmpo proteins in nuclear architecture and cell cycle control is discussed.