Effect of peritoneal fluid from women with endometriosis on implantation in the mouse model
Effect of peritoneal fluid from women with endometriosis on implantation in the mouse model
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DOI：
10.1016/s0015-0282(00)00552-5
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发表时间：
2000-07-01
影响因子：
6.7
通讯作者：
Lessey, BA
中科院分区：
文献类型：
--
作者：
Illera, MJ;Juan, LW;Lessey, BA
目的：通过延迟着床模型研究子宫内膜异位症或非子宫内膜异位症妇女的腹腔液（PF）在小鼠着床中的潜在作用。设计：具有子宫容受性标志物的小鼠实验模型和人PF对着床影响的前瞻性比较。设置：学术性大学和医院项目。干预措施：将从患有和没有子宫内膜异位症的妇女中收集的PF腹膜内注射到最近交配的小鼠中。对处理和未处理动物的着床部位进行计数，并通过原位杂交免疫组织化学法测量妊娠小鼠子宫中的α v β 3整联蛋白。白血病抑制因子和β 3亚单位的α v β 3的北方印迹法测定在怀孕早期和注射PF后。结果：动物接受PF不孕妇女子宫内膜异位症有减少的植入位点的数量相比，动物接受PF生育妇女或最近治疗的子宫内膜异位症患者。在小鼠中，α v β 3和白血病抑制因子的表达在植入时达到峰值，并通过注射来自子宫内膜异位症不孕患者的人PF而降低。结论：白血病抑制因子和α v β 3在小鼠植入时共表达。子宫内膜异位症妇女的PF对胚胎着床有不利影响，可能是通过不利地影响子宫容受性。（Fertil Steril（R）2000;74：41-8. (C)2000年由美国生殖医学协会出版）。
Objective: To investigate the potential role of peritoneal fluid (PF) from women with or without endometriosis in implantation in mice with use of the delayed implantation model.Design: A murine experimental model with markers of uterine receptivity and prospective comparison of the effects of human PF on implantation.Setting: Academic university and hospital program.Intervention(s): PF collected from women with and without endometriosis was injected intraperitoneally into recently mated mice.Main Outcome Measure(s): Implantation sites were counted in treated and untreated animals, and the alpha v beta 3 integrin was measured in the pregnant mouse uterus by immunohistochemistry with in situ hybridization. Leukemia inhibitory factor and the beta 3 subunit of alpha v beta 3 were measured by Northern blot during early pregnancy and after injections of PF.Result(s): Animals receiving PF from infertile women with endometriosis had a reduction in the number of implantation sites compared with animals that received PF from fertile women or from patients with recently treated endometriosis. In the mouse, expression of alpha v beta 3 and leukemia inhibitory factor peaked at the time of implantation and was reduced by injections of human PF from infertile patients with endometriosis.Conclusion(s): Leukemia inhibitory factor and alpha v beta 3 are coexpressed at the time of implantation in the mouse. PF from women with endometriosis has a detrimental effect on embryo implantation, perhaps by adversely affecting uterine receptivity. (Fertil Steril(R) 2000;74:41-8. (C) 2000 by American Society for Reproductive Medicin).