Plasma folate as marker of folate status in epidemiological studies: the European Investigation into Cancer and Nutrition (EPIC)-Potsdam study
Plasma folate as marker of folate status in epidemiological studies: the European Investigation into Cancer and Nutrition (EPIC)-Potsdam study
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DOI：
10.1079/bjn20041211
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发表时间：
2004-09-01
影响因子：
3.6
通讯作者：
Dierkes, J
中科院分区：
文献类型：
--
作者：
Drogan, D;Klipstein-Grobusch, K;Dierkes, J
叶酸缺乏经常被认为是心血管疾病和某些癌症的潜在危险因素。因此，在大规模流行病学研究中对叶酸状况进行可靠评估至关重要。本研究评估了欧洲癌症与营养调查 (EPIC)-波茨坦队列中 363 名参与者的血浆叶酸 (PF) 与红细胞叶酸 (EF) 的价值，作为叶酸状态的标志物。测定 EF 和 PF 总同型半胱氨酸 (tHcy)、吡哆醇、钴胺素、肌酐、总蛋白和压积细胞体积；分析了谷氨酸羧肽酶 (GCP) C1561T、还原叶酸载体 (RFC) G80A 和亚甲基四氢叶酸 (MTHFR) C677T 多态性。进行人体测量并使用 EPIC-Potsdam 食物频率调查问卷评估饮食摄入量。对 EF 和 PF 与可能调节其浓度的因素进行了比较。对五分位数类别中的血液叶酸进行交叉分类导致 75.5% 的所有受试者被正确分类到相同或相邻类别。年龄、BMI、吡哆醇和钴胺素、水果和蔬菜摄入量以及抽血前 24 小时补充维生素与 EF 和 PF 呈正相关。对于 tHcy，发现了反向关联。与具有 677CT 基因型的参与者相比，具有 MTHFR 677TT 基因型的参与者表现出显着升高的 EF 浓度； EF 和 PF 相关性更强（r 0.78，P
Folate deficiency is often discussed as a potential risk factor for CVD and some cancers. Reliable assessment of folate status in large-scale epidemiological studies is therefore of major importance. The present study assessed the value of plasma folate (PF) compared with erythrocyte folate (EF) as a marker of folate status in 363 participants in the European Investigation into Cancer and Nutrition (EPIC)-Potsdam cohort. EF and PF total homocysteine (tHcy), pyridoxine, cobalamin, creatinine, total protein and packed cell volume were determined; glutamate carboxypeptidase (GCP) C1561T, reduced folate carrier (RFC) G80A and methylenetetrahydrofolate (MTHFR) C677T polymorphisms were analysed. Anthropometric measurements were taken and dietary intake was assessed with the EPIC-Potsdam food-frequency questionnaire. Comparison of EF and PF with factors that may modulate their concentrations was performed. Cross-classification of blood folates in quintile categories resulted in correct classification into the same or adjacent category of 75.5% of all subjects. Age, BMI, pyridoxine and cobalamin, fruit and vegetable intake, and vitamin supplementation 24 h before blood draw were positively associated with EF and with PF. For tHcy an inverse association was found. Participants with the MTHFR 677TT genotype showed significantly elevated EF concentrations compared with those with 677CT genotype; EF and PF were more strongly correlated (r 0.78, P