Inhibition of apolipoprotein secretion and phosphatidate phosphohydrolase activity by eicosapentaenoic and docosahexaenoic acids in the perfused rat liver.
Inhibition of apolipoprotein secretion and phosphatidate phosphohydrolase activity by eicosapentaenoic and docosahexaenoic acids in the perfused rat liver.
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DOI：
10.1016/0026-0495(88)90197-7
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发表时间：
1988-12
期刊：
影响因子：
--
通讯作者：
S. Wong;J. Marsh
中科院分区：
文献类型：
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作者：
S. Wong;J. Marsh
将白蛋白结合的二十碳五烯酸(EPA)、二十二碳六烯酸(DHA)或油酸(OA)以维持灌流液浓度为1 mmol/L的速率注入大鼠肝脏2小时，与单纯缺乏脂肪酸的白蛋白相比，DHA或EPA对三酰甘油(TAG)的产量没有显著增加，而油酸(OA)的灌流可使TAG产量增加一倍。DHA或EPA可使载脂蛋白B-100、载脂蛋白B-48、载脂蛋白E和载脂蛋白Cs的标记亮氨酸掺入量减少50%。与油酸或白蛋白单独作用相比，DHA或EPA可使总磷脂磷酸水解酶活性降低35%。在任何情况下，微体和胞质之间的活性分布没有明显的变化。脂肪酸输注对肝脏TAG含量无明显影响。注入的脂肪酸使总肝脂、微粒体脂和分泌的极低密度脂蛋白的脂类得到丰富。分泌型TAG的平均富集度为24%，DHA或EPA为36%。在富含DHA和EPA的海洋油脂饲养实验中，DHA和EPA对PPH活性和载脂蛋白B分泌的影响可能与肝膜脂肪酸组成的变化有关。
Infusion of albumin-bound eicosapentaenoic acid (EPA) docosahexaenoic acid (DHA), or oleic acid (OA) in perfused rat livers was carried out for two hours at a rate that maintained the perfusate concentration at 1 mmol/L. When compared with fatty acid-poor albumin alone, triacylglycerol (TAG) output was not significantly increased with DHA or EPA, whereas OA infusion resulted in a twofold increase. Incorporation of labeled leucine into VLDL apo B-100, apo B-48, apo E, and apo Cs was decreased by 50% by DHA or EPA compared with OA. The total phosphatidate phosphohydrolase activity was decreased by 35% with DHA or EPA compared to oleic acid or albumin alone. In no case was there a significant change in the distribution of activity between the microsomal and cytosolic factions. Fatty acid infusion did not significantly change the liver TAG content. Total liver lipids, microsomal lipids, and lipids of secreted VLDL were enriched with the infused fatty acids. The degree of enrichment for secreted TAG averaged 24% for OA and 36% for DHA or EPA. The effects of DHA and EPA on PPH activity and on apo B secretion in feeding experiments with marine oils rich in these acids may relate to changes in the fatty acid composition of liver membranes.