Fluorescence properties of a new guanosine analog incorporated into small oligonucleotides
Fluorescence properties of a new guanosine analog incorporated into small oligonucleotides
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DOI：
10.1016/s0006-3495(97)78352-8
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发表时间：
1997-12-01
影响因子：
3.4
通讯作者：
Laws, WR
中科院分区：
文献类型：
--
作者：
Driscoll, SL;Hawkins, ME;Laws, WR
测定了3-甲基异黄蝶呤（3-MI）掺入不同寡核苷酸后的荧光性质。这种高荧光鸟苷类似物的吸收和荧光光谱与正常核苷酸和芳香族氨基酸的吸收和荧光光谱很好地分离。将3-MI掺入这些寡核苷酸后，在吸收和荧光发射光谱中观察到的小位移与一般溶剂效应一致，并不表明来自5'端的探针位置、紧邻探针5'或3'端的核苷酸序列或寡聚体的单链或双链性质的任何贡献。然而，稳态和时间分辨荧光研究表明，紧邻探针5'或3'的嘌呤的存在导致单链寡聚体中的一些动态但主要是静态淬灭。此外，对于这些静态淬灭相互作用，3'嘌呤比5'嘌呤更有效，并且腺嘌呤似乎比鸟嘌呤更有效。双链低聚物的形成导致量子产率的额外损失，这也可以主要归因于静态猝灭。这些结果表明，这类新的光谱增强的荧光嘌呤类似物将能够提供有用的信息有关的核酸结构的扰动。
The fluorescence properties of 3-methyl-isoxanthopterin (3-MI) incorporated into different oligonucleotides have been determined. This highly fluorescent guanosine analog has its absorption and fluorescence spectra well resolved from those of the normal nucleotides and the aromatic amino acids. The small shifts observed in absorption and fluorescence emission spectra upon incorporation of 3-MI into these oligonucleotides are consistent with a general solvent effect and do not suggest any contribution from the position of the probe from the 5' end, the sequence of nucleotides immediately 5' or 3' to the probe, or the single-or double-stranded nature of the oligomer. However, steady-state and time-resolved fluorescence studies indicate that the presence of a purine immediately 5' or 3' to the probe results in some dynamic but mostly static quenching in the single-stranded oligomer. Furthermore, a 3' purine is more effective than a 5' purine, and an adenine appears to be more effective than a guanine for these static quenching interactions. Formation of the double-stranded oligomer leads to an additional loss of quantum yield, which can also be ascribed primarily to static quenching. These results show that this new class of spectrally enhanced fluorescent purine analogs will be able to provide useful information concerning the perturbation of nucleic acid structures.