Ethanol extract of Brazilian propolis ameliorates cognitive dysfunction and suppressed protein aggregations caused by hyperhomocysteinemia
Ethanol extract of Brazilian propolis ameliorates cognitive dysfunction and suppressed protein aggregations caused by hyperhomocysteinemia
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DOI：
10.1080/09168451.2015.1056513
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发表时间：
2015-01-01
影响因子：
1.6
通讯作者：
Kanouchi, Hiroaki
中科院分区：
文献类型：
--
作者：
Miyazaki, Yuta;Sugimoto, Yasushi;Kanouchi, Hiroaki
同型半胱氨酸（Hcy）被认为是认知功能障碍的危险因素。研究了蜂胶在体外和体内对hcy诱导的氧化应激的抗氧化作用及其机制。体外实验用Hcy和不同浓度蜂胶培养神经母细胞瘤SH-SY5Y和胶质母细胞瘤U-251MG细胞。与单独使用Hcy相比，蜂胶显著抑制细胞死亡和活性氧产生，且呈剂量依赖性。在体内实验中，小鼠被喂食含有蜂胶的饲料和水中的Hcy硫内酯。采用Morris水迷宫试验评估认知功能。蜂胶抑制高同型半胱氨酸血症引起的认知功能障碍。高同型半胱氨酸血症加速了脑内聚集蛋白的积累，蜂胶抑制了聚集蛋白的积累。然而，高同型半胱氨酸血症并没有增加大脑的氧化应激。体外淀粉样蛋白形成实验表明，Hcy促进溶菌酶的聚集，蜂胶抑制其聚集。
Homocysteine (Hcy) has been proposed to be a risk factor for cognitive dysfunction. We investigated the effects and the underlying mechanisms of action of propolis, which has antioxidant activity on Hcy-induced oxidative stress in vitro and in vivo. For the in vitro assays, neuroblastoma SH-SY5Y and glioblastoma U-251MG cells were cultured with Hcy and various concentrations of propolis. Cell death and reactive oxygen species production were significantly suppressed by propolis in dose-dependent manner, compared with Hcy alone. For the in vivo assays, mice were fed a propolis-containing diet and Hcy thiolactone in water. Cognitive function was evaluated using the Morris water maze test. Propolis suppressed cognitive dysfunction caused by hyperhomocysteinemia. Accumulation of aggregated protein in brain was accelerated in hyperhomocysteinemia, and the accumulation was suppressed by propolis. Hyperhomocysteinemia, however, did not enhance the oxidative stress in brain. In vitro amyloid formation assay showed that Hcy accelerated lysozyme aggregation and propolis inhibited the aggregation.