Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera
Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera
复制标题
新生犊牛腹泻病毒及人血清中和抗体的空斑检测
DOI：
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发表时间：
1977
影响因子：
9.4
通讯作者：
R. Kono
中科院分区：
文献类型：
--
作者：
S. Matsuno;S. Inouye;R. Kono
新生小牛腹泻病毒(一种牛轮状病毒)在已建立的恒河猴肾细胞系MA-104细胞的单层上形成明显的空斑。利用该空斑试验方法，使新生儿腹泻病毒中和抗体的滴定成为可能。结果表明，部分人血清中含有该制剂的中和抗体。
Neonatal calf diarrhea virus (a bovine rotavirus) formed distinct plaques in monolayers of MA-104 cells, an established macacus rhesus monkey kidney cell line, when diethylaminoethyl dextran and trypsin were included in the overlay medium. By using this plaque assay method, titration of neutralizing antibody to neonatal calf diarrhea virus was made feasible. It was demonstrated that some human sera contained neutralizing antibody to this agent.