Penetration of toxic shock syndrome toxin-1 across porcine vaginal mucosa ex vivo: Permeability characteristics, toxin distribution, and tissue damage
Penetration of toxic shock syndrome toxin-1 across porcine vaginal mucosa ex vivo: Permeability characteristics, toxin distribution, and tissue damage
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DOI：
10.1016/s0002-9378(03)00873-1
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发表时间：
2003-12-01
影响因子：
9.8
通讯作者：
Squier, CA
中科院分区：
文献类型：
--
作者：
Davis, CC;Kremer, MJ;Squier, CA
目的：本研究的目的是评估中毒性休克综合征毒素-1的经阴道渗透性及其对离体猪阴道粘膜的渗透性和组织完整性的影响。氚水渗透性（1和10 μ g/mL应用毒素）和S-35-甲硫氨酸标记的中毒性休克综合征毒素-1的跨粘膜通量（10和20 μ g/mL）长达12小时，使用连续流灌注系统进行评估。确定穿透粘膜组织层的标记毒素的位置。毒素处理，完整的，手术刀切割组织的完整性进行了评价tissue-pathologic.Results：中毒性休克综合征毒素-1引起粘膜通透性的非剂量依赖性增加，并穿越完整的粘膜在一个较低的速度，而不破坏组织的完整性。在切开的阴道粘膜，中毒性休克综合征毒素-1诱导上皮下分离和萎缩，类似于临床相关的阴道病变，在月经中毒性休克syndrome.CONCLUSION的致命病例报告：在体外模型可以用来证明，中毒性休克综合征毒素-1渗透阴道粘膜和分布在整个组织。暴露于中毒性休克综合征毒素-1的组织的组织学评价表明，病变与月经中毒性休克综合征病例中报告的病变相似。
OBJECTIVE: The purpose of this study was to evaluate transvaginal penetration of toxic shock syndrome toxin-1 and its effects on permeability and tissue integrity in vitro with the use of excised porcine vaginal mucosa.STUDY DESIGN: Permeability to tritiated water (1 and 10 mug/mL applied toxin) and transmucosal flux of S-35-methionine-labeled toxic shock syndrome toxin-1 (10 and 20 mug/mL) for up to 12 hours were assessed with the use of a continuous flow perfusion system. The location of labeled toxin that penetrated the mucosal tissue strata was determined. The integrity of toxin-treated, intact, scalpel-incised tissue was evaluated histopathologically.RESULTS: Toxic shock syndrome toxin-1 caused a non-dose-dependent increase in mucosal permeability and traversed the intact mucosa at a low rate without disrupting tissue integrity. In incised vaginal mucosa, toxic shock syndrome toxin-1 induced subepithelial separation and atrophy that were analogous to clinically relevant vaginal lesions that were reported in fatal cases of menstrual toxic shock syndrome.CONCLUSION: An in vitro model could be used to demonstrate that toxic shock syndrome toxin-1 permeates the vaginal mucosa and distributes throughout the tissue. Histologic evaluation of tissues that were exposed to toxic shock syndrome toxin-1 demonstrated lesions that were similar to those lesions that were reported in cases of menstrual toxic shock syndrome.