Detection of rhabdovirus viral RNA in oropharyngeal swabs and ectoparasites of Spanish bats
Detection of rhabdovirus viral RNA in oropharyngeal swabs and ectoparasites of Spanish bats
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DOI：
10.1099/vir.0.046490-0
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发表时间：
2013-01-01
影响因子：
3.8
通讯作者：
Echevarria, Juan
中科院分区：
文献类型：
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作者：
Aznar-Lopez, Carolina;Vazquez-Moron, Sonia;Echevarria, Juan
横纹肌病毒感染多种宿主，包括哺乳动物、鸟类、爬行动物、鱼类、昆虫和植物。由于蝙蝠是大多数溶血病毒属成员的天然宿主，因此用于主动监测的扩增方法的特异性通常仅限于溶血病毒。然而，也有报道称在蝙蝠中存在其他横纹肌病毒。为了扩大这种方法的范围，设计了一种新的RT-PCR，能够检测各种各样的横纹肌病毒。该方法检测出86种不同的横纹肌病毒中的81种。总共分析了1488份口咽蝙蝠拭子和38份白蚁样本，检测到17个独特的横纹蛇病毒相关序列。系统发育分析表明，在蝙蝠中检测到的这些序列并不构成单系群，即使它们来自同一种蝙蝠。然而，所有在夜蛾中检测到的序列和从蝙蝠中获得的一个序列确实与黑腹果蝇横纹肌病毒构成单系群。
Rhabdoviruses infect a variety of hosts, including mammals, birds, reptiles, fish, insects and plants. As bats are the natural host for most members of the genus Lyssavirus, the specificity of the amplification methods used for active surveillance is usually restricted to lyssaviruses. However, the presence of other rhabdoviruses in bats has also been reported. In order to broaden the scope of such methods, a new RT-PCR, able to detect a diverse range of rhabdoviruses, was designed. The method detected 81 of 86 different rhabdoviruses. In total, 1488 oropharyngeal bat swabs and 38 nycteribiid samples were analysed, and 17 unique rhabdovirus-related sequences were detected. Phylogenetic analysis suggested that those sequences detected in bats did not constitute a monophyletic group, even when originating from the same bat species. However, all of the sequences detected in nycteribiids and one sequence obtained from a bat did constitute a monophyletic group with Drosophila melanogaster sigma rhabdovirus.