Use of cellular oncogene probes to identify Morone hybrids.
Use of cellular oncogene probes to identify Morone hybrids.
复制标题
使用细胞癌基因探针鉴定 Morone 杂合体。
DOI：
10.1093/oxfordjournals.jhered.a111234
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Mesing,C
中科院分区：
文献类型：
--
作者：
Wirgin,II;Maceda,L;Mesing,C
We tested the ability of cellular oncogene (c-onc) probes to identify F1hybrids and the lineage of known backcrosses within the fish genusMorone. Total DNA was isolated from five to 14 individuals per North AmericanMoronespecies (striped bass, white bass, white perch, and yellow bass). The DNA was digested with two restriction enzymes,EcoRI andHindIII, Southern blotted, and hybridized to six different c-onc probes including v-abl, v-erbB, c-myc, c-H-ras, c-K-ras, and v-src. We found fixed genotypic differences among the four species for all six probes in single restriction enzyme digests. The heritability of these nuclear DNA genotypes was evaluated in hatchery-produced F1Moronhybrids (striped bass ⊠ white bass and striped bass ⊠ white perch) tested with the six informative single probe/restriction enzyme combinations. All F1, individuals exhibited heterozygosity in all diagnostic nuclear DNA fragments, confirming the Mendelian inheritance of these genotypes in these fish. Furthermore, analysis of these nuclear DNA genotypes in hatchery-produced backcrosses of F1hybrids striped bass ⊠ (white bass ⊠ striped bass) detected both recombinant and parental genotypes at all six polymorphic c-onc sequences. The lineage of suspectedMoronehybrids of unknown descent collected from Lewis Smith Lake, Alabama, and from the Occoquan River, Virginia, was determined using the c-onc probes. Our results suggest that c-onc probes are suitable markers to unequivocally identify F1hybrids and backcrosses and to quantify introgression in natural populations of fishes. The addition of RFLP analysis of mtDNA provided a complete ancestral history of individual fish.
