Repressible chloroplast gene expression in Chlamydomonas: A new tool for the study of the photosynthetic apparatus
Repressible chloroplast gene expression in Chlamydomonas: A new tool for the study of the photosynthetic apparatus
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DOI：
10.1016/j.bbabio.2013.11.020
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发表时间：
2014-09-01
影响因子：
4.3
通讯作者：
Rochaix, Jean-David
中科院分区：
文献类型：
--
作者：
Dinc, Emine;Ramundo, Silvia;Rochaix, Jean-David
一个可抑制/诱导的叶绿体基因表达系统已被用来有条件地抑制叶绿体蛋白质合成的单细胞衣藻莱茵衣藻。该系统允许人们在叶绿体翻译停止时跟踪光系统II和光系统I及其触角的命运。主要结果是PSI核心蛋白水平的下降速度比PSII的下降速度慢。在捕光复合物中，CP 26的减少以与PSII核心蛋白相同的速率进行，而CP 29的减少明显较慢，并且对于PSI的天线复合物，该速率介于CP 26和CP 29之间。与此形成鲜明对比的是，三聚体LHCII的主要PSII天线的组件，持续几天后抑制叶绿体翻译。该系统为研究类囊体膜中单个光合复合物的生物合成和周转提供了新的可能性。这篇文章是特刊的一部分，题为：光合作用研究的可持续性：生产清洁能源的关键。(C)2013爱思唯尔有限公司版权所有。
A repressible/inducible chloroplast gene expression system has been used to conditionally inhibit chloroplast protein synthesis in the unicellular alga Chlamydomonas reinhardtii. This system allows one to follow the fate of photosystem II and photosystem I and their antennae upon cessation of chloroplast translation. The main results are that the levels of the PSI core proteins decrease at a slower rate than those of PSII. Amongst the light-harvesting complexes, the decrease of CP26 proceeds at the same rate as for the PSII core proteins whereas it is significantly slower for CP29, and for the antenna complexes of PSI this rate is comprised between that of CP26 and CP29. In marked contrast, the components of trimeric LHCII the major PSII antenna, persist for several days upon inhibition of chloroplast translation. This system offers new possibilities for investigating the biosynthesis and turnover of individual photosynthetic complexes in the thylakoid membranes. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy. (C) 2013 Elsevier B.V. All rights reserved.