Incorporation of [18O]water in the formation of p-hydroxybenzyl alcohol by the p-cresol methylhydroxylase from Pseudomonas putida.
Incorporation of [18O]water in the formation of p-hydroxybenzyl alcohol by the p-cresol methylhydroxylase from Pseudomonas putida.
复制标题
恶臭假单胞菌的对甲酚甲基羟化酶在形成对羟基苯甲醇时掺入[18O]水。
作者：
D. Hopper
在恶臭假单胞菌的酶作用下对甲酚的甲基发生羟基化反应时，氧原子来自于水。虽然由相同的酶进行的第二次反应将产物对羟基苯甲醇转化为醛，但醇是无酶的中间体。
In the hydroxylation of the methyl group of p-cresol by an enzyme from Pseudomonas putida the oxygen atom is derived from water. Although a second reaction by the same enzyme converts the product, p-hydroxybenzyl alcohol, into the aldehyde, the alcohol is an enzyme-free intermediate.