Micropipette measurement of airway submucosal gland secretion. Autonomic effects.
Micropipette measurement of airway submucosal gland secretion. Autonomic effects.
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微量移液器测量气道粘膜下腺分泌物。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
J. A. Nadel
中科院分区：
文献类型：
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作者：
I. Ueki;Victor F. German;J. A. Nadel
我们描述了一种从猫气管体内单个粘膜下腺管中获取分泌物的微量移液管方法。16只动物在基础条件下采集的65个腺体的分泌速率在3 - 30 nl/min之间变化（平均值+/- SE，9.05 +/- 0.60 nl/min），在动物内采集的腺体之间变化2 - 3倍。在10分钟内从单个导管收集的连续样品具有高度可重复性（相关系数为0.96），并且在长达4小时的时间内保持显著恒定。迷走神经冷却使分泌率平均降低39%（n = 11，p <0.05）。刺激颈迷走神经使血流量从8.3 ± 0.7 nl/min增加到16.4 ± 0.8 nl/min（n = 15，p <0.01），静脉注射0.5 mg硫酸阿托品/kg体重或冷却迷走神经可消除该效应，但静脉注射0.08 mg酚妥拉明/kg不能消除该效应。静脉注射0.3 mg苯肾上腺素/kg体重可使血流量从8.6 +/- 0.6增加到18.9 +/- 1.1 nl/min（n = 20，p <0.001），酚妥拉明可阻止这种作用，但阿托品不能。我们得出结论，胆碱能和α-肾上腺素能刺激增加猫粘膜下腺体的液体分泌。
We describe a micropipette method for obtaining secretions from single submucosal gland ducts in vivo in cat tracheas. The secretory rate of 65 glands sampled under basal conditions in 16 animals varied from 3 to 30 nl/min (mean +/- SE, 9.05 +/- 0.60 nl/min) and varied 2 to 3-fold among glands sampled within an animal. Sequential samples collected from individual ducts during a 10-min period were highly repeatable (correlation coefficient, 0.96) and were remarkably constant for as long as 4 h. Vagal cooling decreased the secretory rate by a mean of 39% (n = 11, p less than 0.05). Stimulation of the cervical vagus nerves increased flow from 8.3 +/- 0.7 to 16.4 +/- 0.8 nl/min (n = 15, p less than 0.01), an effect that was abolished by intravenous injection of 0.5 mg of atropine sulfate/kg of body weight or by cooling of the vagus nerves, but not by 0.08 mg of phentolamine/kg given intravenously. Intravenous injection of 0.3 mg of phenylephrine/kg of body weight increased flow from 8.6 +/- 0.6 to 18.9 +/- 1.1 nl/min (n = 20, p less than 0.001), an effect that was prevented by phentolamine, but not by atropine. We conclude that cholinergic and alpha-adrenergic stimulation increases fluid secretion from feline submucosal glands.