CONVERGENTLY FUNCTIONAL, RHO-INDEPENDENT TERMINATOR IN SALMONELLA-TYPHIMURIUM
CONVERGENTLY FUNCTIONAL, RHO-INDEPENDENT TERMINATOR IN SALMONELLA-TYPHIMURIUM
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DOI：
10.1128/jb.163.1.362-368.1985
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
BRUNI, CB
中科院分区：
文献类型：
--
作者：
CARLOMAGNO, MS;RICCIO, A;BRUNI, CB
组氨酸操纵子末端存在一个典型的Rho非依赖性转录终止子。鼠伤寒。除了his操纵子mRNA外，该位点还用于终止在相反链上转录的未知功能的1200个核苷酸的RNA。通过在用于研究调控信号的载体系统中以任一方向克隆终止子结构，在体内和体外研究了在该位点终止转录的效率。在该位点终止转录是非常有效的，在体内和体外，在两个方向。
A typical Rho-independent terminator of transcription was found at the end of the histidine operon of S. typhimurium. This site is used to terminate, in addition to the his operon mRNA, a 1200-nucleotide RNA of unknown function transcribed on the opposite strand. The efficiency of termination of transcription at this site was investigated in vivo and in vitro by cloning of the terminator structure in either orientation in vector systems used to study regulatory signals. Termination of transcription at this site was very efficient, both in vivo and in vitro, and in both orientations.