Intracellular calcium and skin tumor promotion: calcium regulation of the induction of epidermal ornithine decarboxylase activity by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate.
Intracellular calcium and skin tumor promotion: calcium regulation of the induction of epidermal ornithine decarboxylase activity by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate.
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细胞内钙和皮肤肿瘤促进：肿瘤启动子 12-O-十四烷酰佛波醇-13-乙酸酯诱导表皮鸟氨酸脱羧酶活性的钙调节。
DOI：
10.1016/0006-291x(81)91270-5
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发表时间：
1981
影响因子：
3.1
通讯作者：
Boutwell,RK
中科院分区：
文献类型：
--
作者：
Verma,AK;Boutwell,RK
在无血清 Eagle's HeLa 细胞培养基中维持的成年小鼠皮肤外植体中，测定了钙在 12-O-十四烷酰佛波醇-13-乙酸酯 (TPA) 诱导表皮鸟氨酸脱羧酶 (ODC) 中的作用。乙二醇-双-(β-氨基乙基醚) N,N'-四乙酸 (EGTA) 螯合细胞外钙可防止 TPA 诱导 ODC，而 TPA 可以在培养基中钙恢复后恢复 ODC 诱导。细胞外镁不能替代钙用于 TPA 诱导 ODC。皮肤片与钙调蛋白抑制剂三氟拉嗪 (TFP) 的同时孵育抑制了 ODC 的诱导。此外，在介质中加入镧（其对钙结合位点的亲和力高于钙并取代表面结合的钙）抑制了 TPA 对 ODC 的诱导。
The role of calcium in epidermal ornithine decarboxylase (ODC) induction by 12-O-tetradecanoylphorbol-13-acetate (TPA) was determined in adult mouse skin explants maintained in a serum-free Eagle's HeLa cell medium. Chelation of extracellular calcium by ethyleneglycol-bis-(β-aminoethyl ether) N,N′-tetraacetic acid (EGTA) prevented ODC induction by TPA, which could be resumed upon calcium restoration in the medium. Extracellular magnesium could not replace calcium for ODC induction by TPA. Concurrent incubation of skin pieces with a calmodulin inhibitor trifluoperzine (TFP) inhibited ODC induction. Furthermore, inclusion in the medium of lanthanum, which has a higher affinity for calcium-binding sites than calcium and displaces surface-bound calcium, inhibited ODC induction by TPA.