Influence of Hematocrit and Total-Spot Volume on Performance Characteristics of Dried Blood Spots for Newborn Screening.
Influence of Hematocrit and Total-Spot Volume on Performance Characteristics of Dried Blood Spots for Newborn Screening.
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DOI：
10.3390/ijns1020069
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发表时间：
2015
影响因子：
3.5
通讯作者：
De Jesús VR
中科院分区：
文献类型：
--
作者：
Hall EM;Flores SR;De Jesús VR
干血斑（DBS）已用于新生儿筛查（NBS）测试超过50年。疾病控制和预防中心（CDC）的新生儿筛查质量保证计划（NSQAP）进行了研究，以评估红细胞压积和总斑点体积对DBS样本特征的个体影响。每次穿刺的血清体积减少27%，RBC体积增加一倍以上，吸收时间增加300%以上，并且斑点直径在40%至65%的血细胞比容之间略微减小。每冲血清和RBC体积随总斑点体积的降低而递减。患者红细胞压积是一个不可控的变量，不可避免地会影响DBS样本的结果。然而，通过注意DBS的某些物理特性，可以识别出超出可接受范围的样本。
Dried blood spots (DBS) have been used in newborn screening (NBS) tests for over 50 years. The Newborn Screening Quality Assurance Program (NSQAP) at the Centers for Disease Control and Prevention (CDC) conducted studies to assess the individual impacts of hematocrit and total-spot volume on characteristics of DBS samples. Per-punch serum volumes decreased 27%, RBC volumes more than doubled, absorption times increased over 300%, and spot diameters decreased marginally between the hematocrits of 40% to 65%. Per-punch serum and RBC volumes decreased logarithmically with lowering total-spot volumes. Patient hematocrit is an uncontrollable variable and inevitably affects the resulting punch from a DBS sample. It may be possible, though, to identify samples that fall outside of an acceptable range by noting certain physical characteristics of the DBS.