Effect of oxidative stress on AIF-mediated apoptosis and bovine muscle tenderness during postmortem aging
Effect of oxidative stress on AIF-mediated apoptosis and bovine muscle tenderness during postmortem aging
复制标题
氧化应激对死后衰老过程中 AIF 介导的细胞凋亡和牛肌肉嫩度的影响
DOI：
10.1111/1750-3841.14969
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发表时间：
2019-12-09
影响因子：
3.9
通讯作者：
Han, Ling
中科院分区：
文献类型：
--
作者：
Chen, Cheng;Zhang, Jiaying;Han, Ling
本研究旨在探讨氧化应激在牛死后衰老过程中对凋亡诱导因子(AIF)介导的细胞凋亡和牛肌肉嫩度的影响。观察细胞内活性氧(ROS)含量、线粒体膜通透性、AIF表达水平、细胞核凋亡率、剪切力、肌原纤维碎裂指数、pH和能量水平。结果表明，从6小时到72小时，ROS含量的增加伴随着线粒体膜通透性的增加。同时，线粒体AIF的表达在72小时内显著下调。然而，N-乙酰半胱氨酸处理的样品的ROS含量(6-72小时)和线粒体膜通透性(12-72小时)显著低于对照组。此外，在死后老化过程中，线粒体中AIF水平的变化与肉的嫩化和细胞核的凋亡密切相关。这些结果表明，ROS诱导的氧化应激通过提高线粒体膜通透性来显著促进线粒体AIF的释放，释放的AIF介导了细胞核的凋亡，从而进一步增强了牛肌肉的嫩度。此外，结果表明，在早期阶段，环境因素(ATP含量和pH)显著降低(0~72小时)，而ROS诱导的氧化应激对环境因素没有显著影响。这些观察结果进一步表明，在死后老化过程中，AIF的释放需要减少pH和ATP的消耗。我们认为，ROS诱导的氧化应激和内环境通过调控AIF介导的细胞凋亡途径对肉的嫩化起着至关重要的作用。实际应用ROS诱导的氧化应激通过促进细胞凋亡促进牛肌肉嫩化。这可能为通过改变死后肌肉的内部氧化环境来开发创新的嫩化技术奠定理论基础。
This study aimed to explore the effect exerted by oxidative stress on apoptosis-inducing factors (AIF)-mediated apoptosis and bovine muscle tenderness during postmortem aging. We investigated the reactive oxygen species (ROS) content, mitochondrial membrane permeability, AIF expression level, nucleus apoptosis, shear force, myofibril fragmentation index, pH, and energy level. According to the results, a rise in ROS content was accompanied by the rise in mitochondrial membrane permeability from 6 to 72 hr. In the meantime, the AIF expression in mitochondria was downregulated significantly within 72 hr. However, samples treated with N-acetylcysteine had significantly lower ROS content (6 to 72 hr) and mitochondrial membrane permeability (12 to 72 hr) than the control group. Moreover, during postmortem aging, the variations in AIF levels in mitochondria were closely associated with meat tenderization and nucleus apoptosis. These findings demonstrated that oxidative stress induced by ROS significantly promoted AIF release from mitochondria by enhancing the mitochondrial membrane permeability, and the released AIF mediated nucleus apoptosis that further enhanced bovine muscle tenderness. Besides, results suggest that in the early stage, the environmental factors (ATP content and pH) significantly decreased (0 to 72 hr), whereas ROS-induced oxidative stress had no significant effect on environmental factors. These observations further suggested that during postmortem aging, the decrease of pH and ATP consumption are required by AIF release. We conclude that ROS-induced oxidative stress and internal environment are vital for meat tenderization through the regulation of AIF-mediated apoptosis pathway. Practical Application ROS-induced oxidative stress contributes to bovine muscle tenderization by promoting cell apoptosis. It is likely to lay a theoretical foundation for developing innovative tenderization techniques by altering the internal oxidation environment of postmortem muscles.