Noncoding RNAs prevent spreading of a repressive histone mark
Noncoding RNAs prevent spreading of a repressive histone mark
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DOI：
10.1038/nsmb.2619
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发表时间：
2013-08-01
影响因子：
16.8
通讯作者：
Buehler, Marc
中科院分区：
文献类型：
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作者：
Keller, Claudia;Kulasegaran-Shylini, Raghavendran;Buehler, Marc
真核生物基因组的转录比以前预期的更广泛，并导致许多非蛋白质编码RNA（ncRNA）的产生，其功能相关性知之甚少。在这里，我们证明了ncRNA可以抵消异染色质侵入相邻的常染色质。我们已经确定了一个长的ncRNA（称为BORDERLINE），防止传播的HP 1蛋白Swi6和组蛋白H3的Lys9甲基化超出裂殖酵母染色体1的着丝粒周围重复区域。边界RNA以序列独立但基因座依赖的方式起作用，并被Dicer加工成短RNA，称为brdrRNA。与典型的着丝粒短干扰RNA相反，brdrRNA很少加载到Argonaute上。我们的分析揭示了一个意想不到的调节活性的ncRNA在划定一个表观遗传学上不同的染色体结构域，也可以在其他真核生物中操作。
Transcription of eukaryotic genomes is more widespread than was previously anticipated and results in the production of many non-protein-coding RNAs (ncRNAs) whose functional relevance is poorly understood. Here we demonstrate that ncRNAs can counteract the encroachment of heterochromatin into neighboring euchromatin. We have identified a long ncRNA (termed BORDERLINE) that prevents spreading of the HP1 protein Swi6 and histone H3 Lys9 methylation beyond the pericentromeric repeat region of Schizosaccharomyces pombe chromosome 1. BORDERLINE RNAs act in a sequence-independent but locus-dependent manner and are processed by Dicer into short RNAs referred to as brdrRNAs. In contrast to canonical centromeric short interfering RNAs, brdrRNAs are rarely loaded onto Argonaute. Our analyses reveal an unexpected regulatory activity of ncRNAs in demarcating an epigenetically distinct chromosomal domain that could also be operational in other eukaryotes.