Expression of the basal cell markers of taste buds in the anterior tongue and soft palate of the mouse embryo
Expression of the basal cell markers of taste buds in the anterior tongue and soft palate of the mouse embryo
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DOI：
10.1002/cne.21738
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发表时间：
2008-07-10
影响因子：
2.5
通讯作者：
Harada, Shuitsu
中科院分区：
文献类型：
--
作者：
Nakayama, Ayumi;Miura, Hirohito;Harada, Shuitsu
尽管Shh在真菌状乳头基板中的胚胎表达在真菌状乳头的形成中起着关键作用，但其出现是否表明味蕾基底细胞的分化尚不清楚。为了研究基底细胞的胚胎发育，采用原位杂交法测定了小鼠胚胎前舌和软腭中Shh、Prox1和Mash1的表达。在前舌中，在E12.5时，在真菌状乳头基板中Shh开始表达，ProX1与Shh共表达。在E14.5时，Shh在软腭最前部呈带状表达，在后部呈点状表达。观察到Shh的位置（即斑点）数量迅速增加（在E14.5处为21.4 +/- 4.3），并达到峰值（在E15.5处为54.8 +/- 4.0）。同样在软腭中，从Shh表达开始，Prox1就与Shh共表达。这些结果表明基底细胞分化与前舌和软腭的Shh点的模式同步发生。相比之下，Mash1的表达滞后于Shh和Prox1的表达，并且在软腭中Shh位点数量达到峰值后才开始表达。此外，PGP9.5和Shh的免疫组化结果显示，舌头和软腭的上皮神经支配略先于Mash1的表达。这是第一个描述味蕾基底细胞标记物胚胎表达的时间过程的报告。
Although embryonic expression of Shh in the fungiform papilla placodes has a critical role in fungiform, papilla patterning, it remains unclear whether its appearance indicates the differentiation of the basal cells of taste buds. To examine the embryonic development of the basal cells, the expression of Shh, Prox1, and Mash1 was determined in the anterior tongue and soft palate in mouse embryos by in situ hybridization. In the anterior tongue, ProX1 was coexpressed with Shh from the beginning of Shh expression in the fungiform papilla placodes at E12.5. Shh was expressed in the soft palate in a band-like pattern in the anteriormost region and in a punctate pattern in the posterior region at E14.5. The number (21.4 +/- 4.3, at E14.5) of locations where Shh was observed (i.e., spots) rapidly increased and reached a peak level (54.8 +/- 4.0 at E15.5). Also in the soft palate, Prox1 was coexpressed with Shh from the beginning of Shh expression. These results suggest that basal cell differentiation occurs synchronously with the patterning of Shh spots both in the anterior tongue and in the soft palate. In contrast, Mash1 expression lagged behind the expression of Shh and Prox1 and began after the number of Shh spots had reached its peak level in the soft palate. Furthermore, immunohistochemistry of PGP9.5 and Shh revealed that epithelial innervation slightly preceded Mash1 expression both in the tongue and in the soft palate. This is the first report describing the time courses of the embryonic expression of basal cell markers of taste buds.