Effect of prenatal exposure to diethylstilbestrol on gubernacular development in fetal male mice.
Effect of prenatal exposure to diethylstilbestrol on gubernacular development in fetal male mice.
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发表时间：
2004-12
影响因子：
2.9
通讯作者：
Xue-Wu Jiang;Jian-hong Li;Tian-hua Huang;Wang-Dong Deng
中科院分区：
文献类型：
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作者：
Xue-Wu Jiang;Jian-hong Li;Tian-hua Huang;Wang-Dong Deng
目的探讨孕期接触己烯雌酚(DES)对昆明种胎鼠睾丸引带发育的影响及肌动蛋白和增殖细胞核抗原(PC NA)的作用。方法孕鼠随机分为6组，分别于妊娠第9天(E9)至第17天(E17)皮下注射DES，剂量分别为0、25、50、100、200 mg·kg-1·d-1，加入0.2mL二甲基亚砜(DMSO)中。在E17，他们被宰杀，并迅速取出胎儿进行固定。男性胎儿在连续冠状平面上进行切片。光镜下观察组织学变化，扫描和透射电子显微镜观察超微结构变化。免疫组织化学方法检测引带内肌动蛋白和增殖细胞核抗原的表达强度。结果DES组胎鼠死亡率高于DMSO组和生理盐水对照组(P<；0.05)。光镜下可见引带发育不良，鳞茎较小。在扫描电子显微镜下，鳞茎失去了间充质内核和肌肉外层之间的清晰界限，看起来像一个小锥体，而不是正常的圆柱形外观。在透射电子显微镜下，治疗组的引带肌细胞内有一些较小的无序的肌原纤维和稀疏的细胞器。治疗组引带内肌动蛋白和增殖细胞核抗原的表达强度明显弱于二甲基亚砜和生理盐水对照组(P<；0.05)。结论DES以剂量依赖的方式诱导雄性胎鼠引带发育不良，并下调其肌动蛋白和增殖细胞核抗原的表达。
AIM To study the effect of prenatal exposure to diethylstilbestrol (DES) and the role of actin and proliferating cell nuclear antigen (PCNA) on testicular gubernaculum development in fetal male Kunming mice. METHODS Pregnant mice were randomly assigned to 6 groups and injected with DES subcutaneously from gestational day 9 (E9) to day 17 (E17) at doses of 0, 25, 50, 100, 200 microg.kg-1.d-1 in 0.2 mL dimethyl sulfoxide (DMSO). On E17 they were sacrificed and fetuses quickly removed for fixation. Male fetuses were sliced on serial coronal plane. Histological changes were observed under the light microscope (LM) and ultrastructural changes with the scanning and transmission electron microscopes (SEM and TEM). The expression intensity of actin and PCNA in the gubernacula was quantitated by immunohistochemistry. RESULTS The mortality of the fetuses was higher in the DES-treated groups than that in the DMSO and saline controls (P<0.05). Under LM the gubernacula were seen to be poorly developed with smaller bulbs. On SEM the bulbs lose the clear demarcation between the mesenchymal inner core and the muscular outer layer and looked like a small cone instead of the normal cylindrical appearance. On TEM there were some smaller disordered myofibrils and sparse cytoplasmic organelles in the gubernacular muscular cells of the treated groups. The expression intensity of actin and PCNA in the gubernacula was significantly weaker in the treated groups than that in the DMSO and saline controls (P<0.05). CONCLUSION DES induces underdevelopment of the gubernacula in a dose-dependent manner in fetal male mice and down regulates the actin and PCNA expression.