COMPARISON OF 4 PURIFICATION METHODS FOR THE PRODUCTION OF IMMUNOGLOBULINS FROM EGGS LAID BY HENS IMMUNIZED WITH AN ENTEROTOXIGENIC ESCHERICHIA-COLI STRAIN
COMPARISON OF 4 PURIFICATION METHODS FOR THE PRODUCTION OF IMMUNOGLOBULINS FROM EGGS LAID BY HENS IMMUNIZED WITH AN ENTEROTOXIGENIC ESCHERICHIA-COLI STRAIN
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DOI：
10.1016/0022-1759(93)90179-b
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发表时间：
1993-04-02
影响因子：
2.2
通讯作者：
NAKAI, S
中科院分区：
文献类型：
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作者：
AKITA, EM;NAKAI, S
鸡蛋作为特异性抗体来源的重要性是公认的。每毫升蛋黄含有8-20毫克免疫球蛋白（IgY）。然而，分离的主要问题是去除高浓度的脂质。采用水稀释法分离卵黄血浆蛋白与颗粒和脂质。通过盐沉淀和超滤，进一步纯化血浆蛋白中的IgY。将水稀释法（WD）与聚乙二醇（PEG）、硫酸葡聚糖（DS）和黄原胶（Xan）三种方法在产率、纯度、易用性、放大潜力和IgY免疫活性等方面进行了比较。WD法收率最高，DS法次之，Xan法次之，PEG法次之。常规WD法得到的卵黄IgY为9.8 mg /ml，而常用PEG法得到的卵黄IgY为4.9 mg /ml，纯度分别为94%和89%。纯化方法对IgY的免疫活性无不良影响。WD在易于使用和大规模生产IgY方面也有优势。因此，WD法为纯化IgY提供了一种简单、快速、高效、高活性的方法。
The importance of eggs as a source of specific antibodies is well recognized. Egg yolk contains 8-20 mg of immunoglobulins (IgY) per ml. However, the major problem in isolation is removal of lipids which are present in high concentrations. A method had been developed by employing water dilution to separate the yolk plasma proteins from the granules and lipids. Further purification of IgY from plasma proteins was achieved by a protocol involving salt precipitation and ultrafiltration. The water dilution method (WD) was compared with three other methods, namely, polyethylene glycol (PEG), dextran sulphate (DS) and xanthan gum (Xan) in terms of yield, purity, ease of use, potential scaling up and immunoactivity of IgY. The WD method gave the highest yield, followed by DS, Xan and PEG methods in that order. 9.8 mg IgY/ ml egg yolk was routinely obtained from the WD method compared to 4.9 mg IgY/ml egg yolk with the popular PEG method with purities of 94% and 89% respectively. Purification methods had no adverse effect on the immunoactivities of IgY. WD was also found superior in terms of ease of use and large scale production of IgY. WD method therefore provides a simple, rapid and efficient means of purifying IgY with high activity.