Oxygen-glucose deprivation and reoxygenation on human cerebral organoids alters expression related to lipid metabolism
Oxygen-glucose deprivation and reoxygenation on human cerebral organoids alters expression related to lipid metabolism
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DOI：
10.1101/2020.03.31.017335
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发表时间：
2020-03
期刊：
影响因子：
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通讯作者：
N. Iwasa;Takeshi K. Matsui;Naritaka Morikawa;Yoshihiko M. Sakaguchi;T. Shiota;N. Iguchi;Y. Nishimura;Eiichiro Mori;K. Sugie
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文献类型：
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作者：
N. Iwasa;Takeshi K. Matsui;Naritaka Morikawa;Yoshihiko M. Sakaguchi;T. Shiota;N. Iguchi;Y. Nishimura;Eiichiro Mori;K. Sugie
缺血性脑卒中是最常见的神经系统疾病之一。然而，由于缺乏缺血性人脑样本，缺血性卒中对人脑组织的影响在很大程度上仍然未知。在这项研究中，我们使用来自人诱导多能干细胞的脑类器官来评估氧-葡萄糖剥夺/再氧合（OGD/R）的影响。我们鉴定了15个差异表达基因（DEG），发现所有DEG均下调。途径分析显示维生素消化吸收、脂肪消化吸收、过氧化物酶体增殖物激活受体信号通路、补体和凝血级联反应之间的关系。这些结果表明，在人类脑组织缺血性损伤的机制。
Ischemic stroke is one of the most common neurological disease. However, the impact of ischemic stroke on human cerebral tissue remains largely unknown; due to a lack of ischemic human brain samples. In this study, we used cerebral organoids derived from human induced pluripotent stem cells to evaluate the effect of oxygen-glucose deprivation/reoxygenation (OGD/R). We identified 15 differentially expressed genes (DEGs); and found that all the DEGs were downregulated. Pathway analysis showed the relationship of vitamin digestion and absorption, fat digestion and absorption, peroxisome proliferator-activated receptor signaling pathway, and complement and coagulation cascades. These findings indicate the mechanisms underlying ischemic injury in human cerebral tissue.