Monitoring of the early molecular resistance responses of coffee (Coffea arabica L.) to the rust fungus (Hemileia vastatrix) using real-time quantitative RT-PCR
Monitoring of the early molecular resistance responses of coffee (Coffea arabica L.) to the rust fungus (Hemileia vastatrix) using real-time quantitative RT-PCR
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DOI：
10.1016/j.plantsci.2005.12.009
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发表时间：
2006-06-01
期刊：
影响因子：
5.2
通讯作者：
Fernandez, D.
中科院分区：
文献类型：
--
作者：
Ganesh, D.;Petitot, A. -S.;Fernandez, D.
咖啡（Coffea arabica L.）对橙子锈菌Hemileia vastatrix的基因表达进行实时荧光定量RT-PCR分析。在不亲和互作中，接种后12-16 h可观察到真菌感染对咖啡基因的显著激活。显微镜观察表明，在这个时候，只有有限数量的真菌胚芽已经分化出穿透菌丝通过气孔。CaWRKY 1基因的激活，puridine编码的WRKY转录因子也发生在兼容的相互作用，但被延迟到24 hpi。相反，激活的CaR 111基因编码的蛋白质的未知功能只发生在不相容的相互作用。CaNDR 1基因是拟南芥非小种特异性抗病基因（ndr 1）的同源基因，真菌感染只能诱导很差。创伤和水杨酸处理显著激活CaWRKY 1、CaNDR 1和CaR 111基因表达。这些结果表明，在H. vastatrix进入叶片的过程已经发生，并表明激活的基因在咖啡对锈菌的分子抗性反应中可能起作用。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Molecular resistance responses of coffee (Coffea arabica L.) to the orange rust fungus Hemileia vastatrix were monitored by real-time quantitative RT-PCR analysis of gene expression. Significant activation of coffee genes by fungal infection could be observed around 12-16 h post inoculation (hpi) in the incompatible interaction. Microscopic observations indicated that, at this time, only a limited number of fungal germlings already differentiated a penetration hypha through the stomata. Activation of the CaWRKY1 gene, putatively encoding a WRKY transcription factor also occurred in the compatible interaction, but was delayed to 24 hpi. In contrast, activation of the CaR111 gene encoding a protein of unknown function only occurred in the incompatible interaction. The CaNDR1 gene, an homolog of the Arabidopsis non-race specific disease resistance (ndr1) gene was only poorly induced by fungal infection. Wounding and salicylic acid treatment markedly activated CaWRKY1, CaNDR1 and CaR111 gene expression. These results showed that specific transcriptional responses of coffee were detected before penetration of H. vastatrix into the leaf had occurred, and suggest a possible role of activated genes in the molecular resistance responses of coffee to the rust fungus. (c) 2005 Elsevier Ireland Ltd. All rights reserved.