Enhancement of neurite outgrowth using nano-structured scaffolds coupled with laminin
Enhancement of neurite outgrowth using nano-structured scaffolds coupled with laminin
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DOI：
10.1016/j.biomaterials.2008.05.014
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发表时间：
2008-09-01
期刊：
影响因子：
14
通讯作者：
Ramakrishna, S.
中科院分区：
文献类型：
--
作者：
Koh, H. S.;Yong, Thomas;Ramakrishna, S.
在损伤组织的修复和再生过程中，细胞与支架的相互作用对细胞和组织的发育具有重要意义。模拟细胞外基质(ECM)表面形貌、机械硬度和化学组成的支架将有利于促进增强的细胞相互作用。电纺可以很容易地生产出结构类似于组织ECM的纳米结构合成聚合物垫。虽然电纺可以生产亚微米纤维支架，但用生物活性分子修饰电纺支架是有益的，因为这可以创造一个由生化线索组成的环境，进一步促进细胞黏附、增殖和分化。将层粘连蛋白(一种促进细胞外基质的轴突蛋白)掺入纳米纤维是进一步模拟神经组织的生化特性以创建仿生支架的替代方案。在这项研究中，我们研究了通过将层粘连蛋白偶联到聚L-乳酸(PLLA)纳米纤维上来功能化支架的可行性。通过共价结合、物理吸附或共混电纺法成功地将层粘连蛋白添加到纳米纤维中。PC12细胞活性和轴突生长分析证实，功能化的纳米纤维能够增强轴突延伸。值得注意的是，与共价固定化和物理吸附相比，层粘连蛋白与合成聚合物的混合电纺丝是一种简单而有效的修饰纳米纤维的方法，用于构建仿生支架。利用这些功能化技术，可以有效地用层粘连蛋白修饰纳米纤维，使其在周围神经再生中具有潜在的应用前景。(C)2008爱思唯尔有限公司。保留所有权利。
Cell interactions with scaffolds are important for cell and tissue development in the process of repairing and regeneration of damaged tissue. Scaffolds that mimic extracellular matrix (ECM) surface topography, mechanical stiffness, and chemical composition will be advantageous to Promote enhanced cell interactions. Electrospinning can easily produce nano-structured synthetic polymer mats with architecture that structurally resembles the ECM of tissue. Although electrospinning can produce sub-micron fibrous scaffolds, modification of electrospun scaffolds with bioactive molecules is beneficial as this can create an environment that consists of biochemical cues to further promote cell adhesion, proliferation and differentiation. Incorporation of laminin, a neurite promoting ECM protein, onto the nanofibers is an alternative to further mimic the biochemical properties of the nervous tissue to create a biomimetic scaffold. In this study, we investigated the feasibility to functionalize scaffolds by coupling laminin onto poly(L-lactic acid) (PLLA) nanofibers. Laminin was successfully added to nanofibers using covalent binding, physical adsorption or blended electrospinning procedures. PC12 cell viability and neurite outgrowth assays confirmed that the functionalized nanofibers were able to enhance axonal extensions. Significantly, compared to covalent immobilization and physical adsorption, blended electrospinning of laminin and synthetic polymer is a facile and efficient method to modify nanofibers for the fabrication of a biomimetic scaffold. Using these functionalization techniques, nanofibers can be effectively modified with laminin for potential use in peripheral nerve regeneration applications. (c) 2008 Elsevier Ltd. All rights reserved.