An antiviral state induced in Chinook salmon embryo cells (CHSE-214) by transfection with the double-stranded RNA poly I:C
An antiviral state induced in Chinook salmon embryo cells (CHSE-214) by transfection with the double-stranded RNA poly I:C
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DOI：
10.1006/fsim.2002.0412
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发表时间：
2002-11-01
影响因子：
4.7
通讯作者：
Robertsen, B
中科院分区：
文献类型：
--
作者：
Jensen, I;Larsen, R;Robertsen, B
I型干扰素（IFN α和IFN β）通过诱导抑制病毒复制的蛋白质的表达将脊椎动物细胞转化为抗病毒状态。在人类和小鼠中，Mx蛋白构成干扰素诱导的抗病毒蛋白的一个家族。Mx基因最近已经从大西洋鲑鱼和虹鳟鱼中克隆出来。此外，双链RNA（dsRNA）和I型IFN样活性已显示在鲑鱼细胞中诱导Mx蛋白。已经表明奇努克鲑鱼胚胎细胞（CHSE-214细胞）在IFN系统中具有缺陷，因为dsRNA聚肌苷酸聚胞苷酸（poly I：C）未能在细胞中诱导抗病毒状态。在本书中，我们更仔细地研究了这一现象。CHSE-214细胞用poly I：C转染或在没有转染试剂的情况下与poly I：C孵育。然后研究细胞的Mx蛋白表达和对感染性胰腺坏死病毒（IPNV）感染的保护。结果表明，用poly I：C转染的细胞被保护免受IPNV感染，而用poly I：C孵育的细胞不受保护。用双链DNA poly dI：dC转染的细胞也不受IPNV的保护。Mx蛋白表达CHSE-214细胞后，转染与聚I：C，但不与聚I：C单独孵育。用来自用poly I：C转染的细胞的上清液刺激CHSE-214细胞，诱导针对IPNV的保护，表明产生I型IFN样活性。这些结果表明，CHSE-214细胞实际上能够产生I型IFN，但可能在介导poly I：C摄取的机制中存在缺陷，或可能降解未保护的poly I：C。(C)2002爱思唯尔科技有限公司。保留所有权利。
Type I interferons (IFN alpha and beta) convert vertebrate cells into an antiviral state by inducing expression of proteins that inhibit virus replication. In humans and mice, Mx proteins constitute one family of interferon-induced antiviral proteins. Mx genes have recently been cloned from Atlantic salmon and rainbow trout. Moreover, double-stranded RNA (dsRNA) and type I IFN-like activity have been shown to induce Mx protein in salmonid cells. Chinook salmon embryo cells (CHSE-214 cells) have been suggested to have a defect in the IFN-system because the dsRNA polyinosinic polycytidylic acid (poly I:C) failed to induce an antiviral state in the cells. We have studied this phenomenon more closely in the present work. CHSE-214 cells were either transfected with poly I:C or incubated with poly I:C without transfection reagent. The cells were then studied for Mx protein expression and protection against infectious pancreatic necrosis virus (IPNV) infection. The results showed that cells transfected with poly I:C were protected from IPNV infection, whilst cells incubated with poly I:C were not protected. Cells transfected with the double-stranded DNA poly dI:dC were also not protected against IPNV. Mx protein was expressed in CHSE-214 cells upon transfection with poly I:C, but not after incubation with poly I:C alone. Stimulation of CHSE-214 cells with supernatants from cells transfected with poly I:C, induced protection against IPNV, indicating production of type I IFN-like activity. These results suggest that CHSE-214 cells in fact are able to produce type I IFN, but may have defects in the mechanisms mediating uptake of poly I:C or may degrade unprotected poly I:C. (C) 2002 Elsevier Science Ltd. All rights reserved.