Self referencing ceramic based multisite microelectrodes for the detection and elimination of interferences from the measurement of L-glutamate and other analytes
Self referencing ceramic based multisite microelectrodes for the detection and elimination of interferences from the measurement of L-glutamate and other analytes
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DOI：
10.1021/ac0010429
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发表时间：
2001-03-01
影响因子：
7.4
通讯作者：
Gerhardt, GA
中科院分区：
文献类型：
--
作者：
Burmeister, JJ;Gerhardt, GA
采用基于陶瓷的多位点阵列上的两个微电极的自参考技术来确认和消除基于酶的微电极检测到的干扰。测试系统采用谷氨酸氧化酶测定L-谷氨酸；然而，可以使用其他氧化酶，例如葡萄糖氧化酶。一个记录位点涂有带有L-谷氨酸氧化酶和与戊二醛交联的牛血清白蛋白（BSA）的Nafion，而另一个记录位点则涂有带有与戊二醛交联的BSA的Nafion。两个记录位点的化学差异允许识别和消除要从分析物响应中去除的干扰信号。该电极在体外校准 L-谷氨酸时表现出低检测限（LOD = 0.98 +/- 0.09 muM，信噪比为 3）、快速响应（T-90 类似于 1 s）以及在 0-200 muM 浓度范围内出色的线性度（R-2 = 0.999 +/- 0.000）。多位点电极的选择性和尺寸允许体内谷氨酸测量。该电极已应用于体内测量麻醉大鼠前额皮质中局部应用的谷氨酸的清除率和谷氨酸的释放。此外，类似的方法已应用于开发用于测量葡萄糖的微电极。
A self-referencing technique utilizing two microelectrodes on a ceramic-based multisite array is employed for confirmation and elimination of interferences detected by enzyme-based microelectrodes. The measurement of L-glutamate using glutamate oxidase was the test system; however, other oxidase enzymes such as glucose oxidase can be employed. One recording site was coated with Nafion with L-glutamate oxidase and bovine serum albumin (BSA) cross-linked with glutaraldehyde while the other had Nafion with BSA cross-linked with glutaraldehyde. Differences in the chemistry of the two recording sites allowed for identification and elimination of interfering signals to be removed from the analyte response. The electrode showed low detection limits (LOD = 0.98 +/- 0.09 muM, signal-to-noise ratio of 3), fast response tithes (T-90 similar to1 s), and excellent linearity (R-2 = 0.999 +/- 0.000) over the concentration range of 0-200 muM for calibrations of L-glutamate in vitro. The selectivity and dimensions of the multisite electrode allow in vivo glutamate measurements. This electrode has been applied to in vivo measurements of the clearance of locally applied glutamate and release of glutamate in the prefrontal cortex of anesthetized rats. In addition, a similar approach has been applied to the development of a microelectrode for measures of glucose.