IMMUNOSPECIFIC NMR CONTRAST AGENTS
IMMUNOSPECIFIC NMR CONTRAST AGENTS
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DOI：
10.1016/0730-725x(86)91045-3
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发表时间：
1986-01-01
影响因子：
2.5
通讯作者：
LEIGH H S JR
中科院分区：
文献类型：
--
作者：
RENSHAW P F;OWEN C S;LEIGH H S JR
迄今为止，大多数核磁共振造影剂都是顺磁性的。这些试剂包括过渡金属离子和稀土金属离子以及稳定的有机自由基，在远低于1 mM的浓度下不能提供有效的对比。然而，大分子铁磁性和超顺磁性颗粒的使用首次提供了一种在亚纳摩尔浓度下有效的核磁共振弛豫剂。两个不同大小的超顺磁性颗粒被连接到针对神经母细胞瘤特异性细胞表面抗原的高亲和力的单抗上。证明了这些颗粒在体内和体外的特异性结合，并讨论了免疫特异性核磁共振对比的结果。
Most NMR contrast agents suggested to date have been paramagnetic. These agents, which include the transition and lanthanide metal ions as well as stable organic free radicals, do not provide effective contrast at concentrations much below 1 mM. However, the use of macromolecular ferromagnetic and superparamagnetic particles provides, for the first time, an NMR relaxation agent that is effective at subnanomolar concentrations. Two different sized superparamagnetic particles have been coupled to monoclonal antibodies with high affinity for a neuroblastoma-specific cell surface antigen. The specific binding of these particles, both in vivo and in vitro is demonstrated and the consequences for immunospecific NMR contrast are discussed.