TREM-1, MDL-1, and DAP12 expression is associated with a mature stage of myeloid development
TREM-1, MDL-1, and DAP12 expression is associated with a mature stage of myeloid development
复制标题
DOI：
10.1016/s0161-5890(02)00004-4
复制
发表时间：
2002-03-01
影响因子：
3.6
通讯作者：
Margolin, JF
中科院分区：
文献类型：
--
作者：
Gingras, MC;Lapillonne, H;Margolin, JF
髓细胞上表达的触发受体（TREM-1）和髓细胞DAP12相关凝集素（MIDL-1）是最近发现的两种受体，它们与DAP12非共价结合，形成参与单核细胞活化和炎症反应的受体复合物。在本研究中，我们研究TREM-1、MDL-1和DAP12的表达是否与骨髓单核细胞分化相关。Northern和RT-PCR结果显示，与未分化的骨髓CD34（+）干细胞相比，TREM-1、MDL-1和DAP12在外周血源性CD14（+）成熟单核细胞以及分化与未分化的U937细胞中表达强烈。TREM-1和MDL-1 RNA在成人组织中的表达高于胎儿组织，正常细胞中的表达高于恶性细胞。这些发现提示TREM-1/DAP12和MDL-1/DAP12信号通路是成熟分化的骨髓单核细胞的特征。此外，还检测到另一种mRNA TREM-1剪接变体（TREM-1sv）的表达，该变体可能转化为一种可溶性受体，具有作为髓细胞活化调节剂的潜力。(C) 2002 Elsevier Science Ltd.版权所有。
The triggering receptor expressed on myeloid cells (TREM-1) and the myeloid DAP12-associating lectin (MIDL-1) are two recently identified receptors which associate non-covalently with DAP12 to form receptor complexes involved in monocytic activation and inflammatory response. In this study, we investigated whether the expression of TREM-1, MDL-1, and DAP12 correlated with myelomonocytic differentiation. Northern and RT-PCR revealed a strong expression of TREM-1, MDL-1, and DAP12 in peripheral blood-derived CD14(+) mature monocytes in contrast to undifferentiated bone marrow CD34(+) stem cells, and in the differentiated versus undifferentiated U937 cells. TREM-1 and MDL-1 RNA expression was also more elevated in adult than fetal tissues and in normal than malignant cells. These findings suggest that the TREM-1/DAP12 and MDL-1/DAP12 signaling pathways are features of mature differentiated myelomonocytic cells. In addition, expression of an alternative mRNA TREM-1 splice variant (TREM-1sv) was detected that might translate into a soluble receptor with potential as a regulator of myeloid activation. (C) 2002 Elsevier Science Ltd. All rights reserved.