The expression of leptin and its receptors in pre-ovulatory human follicles
The expression of leptin and its receptors in pre-ovulatory human follicles
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DOI：
10.1093/molehr/3.6.467
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发表时间：
1997-06-01
影响因子：
4
通讯作者：
Snodgrass, HR
中科院分区：
文献类型：
--
作者：
Cioffi, JA;VanBlerkom, J;Snodgrass, HR
通过逆转录聚合酶链反应和免疫荧光检测了瘦素及其受体在排卵前卵泡颗粒和卵囊细胞以及体外受精妇女的减数分裂成熟卵母细胞中的表达，并测量了在给予排卵剂量的人绒毛膜促性腺激素前后新抽吸的卵泡液和母亲血清中的瘦素浓度。研究结果表明，瘦素在颗粒细胞和卵丘细胞的mRNA和蛋白水平上表达，并且在成熟的人卵母细胞中存在瘦素。虽然未观察到卵泡瘦素浓度与胚胎发育之间的关联，但排卵后血清瘦素浓度的增加与着床潜力有关。这些结果讨论了瘦素在早期人类发育中的可能作用。
The expression of leptin and its receptors was examined by reverse transcriptase-polymerase chain reaction and immunofluorescence in granulosa and cumulus cells of pre-ovulatory follicles and in meiotically mature oocytes obtained from women undergoing in-vitro fertilization, Leptin concentrations were measured in newly aspirated follicular fluids and in maternal serum before and after the administration of an ovulatory dose of human chorionic gonadotrophin, The findings demonstrate leptin expression at the mRNA and protein levels by granulosa and cumulus cells, and the presence of leptin in mature human oocytes. While an association between follicular leptin concentration and embryo development was not observed, a postovulatory increase in serum leptin concentration was associated with implantation potential. The results are discussed with respect to possible roles of leptin in early human development.