MRI detection of early bone metastases in B16 mouse melanoma models
MRI detection of early bone metastases in B16 mouse melanoma models
复制标题
DOI：
10.1007/s10585-005-1264-9
复制
发表时间：
2005-09-01
影响因子：
4
通讯作者：
Weilbaecher, K
中科院分区：
文献类型：
--
作者：
Gauvain, KM;Garbow, JR;Weilbaecher, K
骨转移导致癌症患者的显著发病率，包括骨痛、病理性骨折、神经压迫综合征和高钙血症。动物模型被用来研究骨转移的发病机制和评估潜在的治疗药物。以前发表的啮齿动物模型中骨转移的成像方法主要集中在使用简单的X射线来识别晚期转移。在此，我们报告了MRI作为一种检测活体小鼠模型早期骨转移的方法。将B16小鼠黑色素瘤细胞注入C57BL/6小鼠的左心室，在组织学上可见肿瘤相关的骨质破坏，但在X射线下看不到肿瘤相关的骨质破坏，在此基础上获得了左小腿的磁共振图像。测定健康对照小鼠和B16黑色素瘤荷瘤小鼠骨髓的T1和T2弛豫时间。正常骨髓T2值平均为28ms(SD5)，病变骨髓T2值平均为41ms(SD3)。病变骨髓T2弛豫时间明显长于正常骨髓(P
Bone metastasis causes significant morbidity in cancer patients, including bone pain, pathologic fractures, nerve compression syndrome, and hypercalcemia. Animal models are utilized to study the pathogenesis of skeletal metastases and to evaluate potential therapeutic agents. Previously published methods for imaging bone metastasis in rodent models have focused on identifying advanced stage metastasis using simple X-rays. Here we report MRI as a method for detecting early bone metastases in mouse models in vivo. B16 mouse melanoma cells were injected into the left cardiac ventricle of C57BL/6 mice and magnetic resonance (MR) images were obtained of the left leg following the development of metastatic disease, when tumor associated bone destruction was histologically present but not visible by X-ray. T1 and T2 relaxation times of bone marrow were measured in healthy control mice and B16 melanoma tumor-bearing mice. Mean T2 values for normal marrow were 28 ms (SD 5) and for diseased bone marrow were 41 ms (SD 3). T2 relaxation time of diseased bone marrow is significantly longer than that of normal bone marrow (P