Intraperitoneal Administration of Plasma-Activated Medium: Proposal of a Novel Treatment Option for Peritoneal Metastasis From Gastric Cancer
Intraperitoneal Administration of Plasma-Activated Medium: Proposal of a Novel Treatment Option for Peritoneal Metastasis From Gastric Cancer
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DOI：
10.1245/s10434-016-5759-1
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发表时间：
2017-05-01
影响因子：
3.7
通讯作者：
Kodera, Yasuhiro
中科院分区：
文献类型：
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作者：
Takeda, Shigeomi;Yamada, Suguru;Kodera, Yasuhiro
非平衡大气压等离子体(NEAPP)照射的液体给药作为一种治疗癌症的新方法引起了人们的极大兴趣。作者先前报道了等离子体激活介质(PAM)通过诱导细胞凋亡来治疗癌细胞系的疗效。本研究通过建立小鼠腹膜转移模型，对PAM的体内治疗效果进行了评价，并对两种胃癌细胞株进行了增殖实验，通过改变血浆源到培养基面的距离和改变照射介质的体积来优化PAM的产生。通过创面愈合和黏附实验，观察PAM对细胞迁移和黏附能力的影响。最后，采用增强绿色荧光蛋白标记的小鼠胃癌细胞模型，探讨了PAM对腹膜转移的抑制作用，较短的血浆源距离和较小的处理介质体积增加了PAM的抗肿瘤作用。PAM在体外可抑制胃癌细胞的迁移和黏附。在小鼠腹膜转移模型中，PAM可使腹膜转移结节的形成减少60%，且未观察到不良反应。血浆活化液有望成为治疗胃癌腹膜转移的一种新方法。
The administration of fluid irradiated with non-equilibrium atmospheric pressure plasma (NEAPP) has attracted much interest as a novel therapeutic method for cancer. The authors previously reported on the efficacy of plasma-activated medium (PAM) for treating cancer cell lines through the induction of apoptosis. In this study, the therapeutic effect of PAM was evaluated in vivo using a peritoneal metastasis mouse model.Two gastric cancer cell lines were used in proliferation assays performed to optimize the production of PAM by changing the distance between the plasma source and the medium surface and by altering the volume of irradiated medium. Wound-healing and adhesion assays were conducted to determine the effect of PAM therapy on cell migration and adhesion capacity in vitro. Finally, a mouse model established by the intraperitoneal injection of enhanced green fluorescent protein-tagged gastric cancer cells was used to explore the efficacy of PAM administered intraperitoneally in inhibiting peritoneal metastasis formation.Shorter distances between the plasma source and the medium surface and smaller volumes of treated medium increased the anti-tumor effect of PAM. The PAM treatment attenuated gastric cancer cell migration and adhesion in vitro. The intraperitoneal administration of PAM decreased the formation of peritoneal metastatic nodules by 60% in the mouse model, and no adverse events were observed.Plasma-activated liquids may represent a novel therapeutic method for the treatment of peritoneal metastases in gastric cancer.