Stress-induced memory alters growth of clonal off spring of white clover (Trifolium repens)
Stress-induced memory alters growth of clonal off spring of white clover (Trifolium repens)
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DOI：
10.3732/ajb.1500526
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发表时间：
2016-09-01
影响因子：
3
通讯作者：
Latzel, Vit
中科院分区：
文献类型：
--
作者：
Gonzalez, Alejandra Pilar Rendina;Chrtek, Jindrich;Latzel, Vit
研究前提：一个个体的表型可以被其前辈所经历的环境所改变，这种现象称为代际效应或母性效应。这些影响主要是在有性世代之间研究的，并且被认为也是由表观遗传变异介导的。然而，我们不知道如何重要的跨无性世代的无性系plants.METHODS：我们调查了不同的干旱强度和持续时间经历的亲本植株的三叶草的无性系扦插移植后，控制条件下的后代分株的生长的作用。我们还处理了一半的植物与5-氮胞苷，这是一种去甲基化剂，以测试潜在的作用DNA甲基化的transgenerational effects.KEY结果：Transgenerational效应表现为后代分株的生物量增加，如果亲本植物经历了中等干旱适用于短时间内和后代分株的生物量下降，如果亲本植物经历了强烈的干旱短时间内。这些跨代效应没有观察到后代的父母从相同的处理，如果这些处理与5-氮杂胞苷，其应用显着降低的5-甲基-2 '-脱氧胞苷在plants.CONCLUSIONS：跨代效应可能发挥重要作用，在克隆植物白三叶，可能是介导的表观遗传变异。克隆植物的生长和行为不仅受到周围环境的影响，而且还受到克隆繁殖时不再存在的环境的影响。这一现象对克隆植物的生态学和进化有着尚未被承认的影响。
PREMISE OF THE STUDY: The phenotype of an individual can be modified by the environment experienced by its predecessors, a phenomenon called transgenerational or maternal effects. These effects are studied mostly across sexual generations and are thought to be mediated also by epigenetic variation. However, we do not know how important transgenerational effects are across asexual generations of clonal plants.METHODS: We investigated the role of different drought intensities and durations experienced by parental plants of Trifolium repens on the growth of off spring ramets after transplantation of clonal cuttings to control conditions. We also treated half of the plants with 5-azacytidine, which is a demethylating agent, to test the potential role of DNA methylation on transgenerational effects.KEY RESULTS: Transgenerational effects were manifested as increased biomass of off spring ramets if parental plants experienced medium drought applied for a short period and decreased biomass of off spring ramets if parental plants experienced intense drought for a short period. These transgenerational effects were not observed for off spring of parents from the same treatments if these were treated with 5-azacytidine, whose application significantly decreased the amount of 5-methyl-2'-deoxycytidine in plants.CONCLUSIONS: Transgenerational effects might play an important role in the clonal plant Trifolium repens and are probably mediated by epigenetic variation. The growth and behavior of clonal plants might be affected not only by the ambient environment but also by environments that are no longer present at the time of clonal reproduction. This phenomenon can have yet unacknowledged ecological and evolutionary implications for clonal plants.