Evolutionary diverse Chlamydomonas reinhardtii Old Yellow Enzymes reveal distinctive catalytic properties and potential for whole-cell biotransformations
Evolutionary diverse Chlamydomonas reinhardtii Old Yellow Enzymes reveal distinctive catalytic properties and potential for whole-cell biotransformations
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DOI：
10.1016/j.algal.2020.101970
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发表时间：
2020-09-01
影响因子：
5.1
通讯作者：
Happe, Thomas
中科院分区：
文献类型：
--
作者：
Bohmer, Stefanie;Marx, Christina;Happe, Thomas
Old Yellow酶（OYEs）具有良好的立体选择性，可选择性地还原多种底物的碳-碳双键。它们在生物基工业过程中应用的当前挑战是烟酰胺共底物的成本效益再生和扩大工业相关底物的范围。微藻是一种丰富且尚未开发的OYEs来源，具有新颖的催化特性，是通过光合作用再生烟酰胺的有前途的全细胞工厂。通过比较系统发育，我们在11种藻类中鉴定出20个假定的OYEs。来自莱茵衣藻（Chlamydomonas reinhardtii, CrOYEs）的3个重组OYEs显示出不同的生物催化特性和酶优化潜力。野生型和突变型活藻细胞的高底物转化活性将生物催化谱与体内功能联系起来，并证明了微藻作为独特的OYE活性和光驱动生物转化来源的潜力。
Old Yellow Enzymes (OYEs) selectively reduce carbon-carbon double bonds of a broad range of substrates with excellent stereoselectivity. Current challenges of their application in bio-based industrial processes are the cost-efficient regeneration of the nicotinamide co-substrate and expanding the range of industrially relevant substrates. Microalgae represent a rich and mostly untapped source for OYEs with novel catalytic properties and are promising whole-cell factories that regenerate nicotinamide by photosynthesis. By comparative phylogenetics, we identified 20 putative OYEs in eleven algal species. Three recombinant OYEs from the unicellular green alga Chlamydomonas reinhardtii (CrOYEs) reveal diverse biocatalytic properties and potential for enzyme optimization. High substrate conversion activities of living algal wild type and mutant cells linked the biocatalytic profiles to in vivo functionality and demonstrate the potential of microalgae as sources for distinctive OYE activities and for light-driven biotransformations.