Changes in bioactive proteins and serum proteome of human milk under different frozen storage
Changes in bioactive proteins and serum proteome of human milk under different frozen storage
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不同冻藏条件下母乳生物活性蛋白及血清蛋白质组的变化
DOI：
10.1016/j.foodchem.2021.129436
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发表时间：
2021
期刊：
影响因子：
8.8
通讯作者：
Zhou Peng
中科院分区：
文献类型：
--
作者：
Qu Jingyan;Zhang Lina;Yin Li'ang;Liu Jun;Sun Zhaona;Zhou Peng
本研究利用−标记蛋白质组学技术和酶联免疫吸附试验试剂盒，研究了人乳在不同冷冻条件下(−18℃和60℃，60d和180d)中常量营养素、细菌总数和血清蛋白质组的变化。结果表明，在−温度为18℃时，总蛋白浓度和需氧细菌总数显著降低，而在−温度为60℃时差异不显著。−18°C贮藏对HM血清蛋白质谱的影响远大于−60°C贮藏对HM血清蛋白质谱的影响。−18°C下乳脂球膜蛋白的增加与乳脂球膜蛋白的损伤和转移密切相关。生物活性蛋白的减少可能与冰变性有关。综上所述，快速降温和超低恒温更适合于人乳的冷冻保存。
This study aimed to investigate changes in macronutrients, total bacterial count, and serum proteome of human milk (HM) under different frozen storage (−18°C and −60°C, 60 d and 180 d) by using IBT Labeling proteomics techniques and ELISA kit. The results indicated that total protein concentrations and total aerobic bacterial counts were significantly decreased at −18°C, while no difference at −60°C. A total of 1617 proteins were identified and quantified, and 173 proteins were significantly different. The −18°C storage had much higher influence on HM serum protein profiles than that of −60°C. Increased milk fat globule membrane (MFGM) proteins at −18°C are highly related to the damage of MFGM and transfer of MFGM proteins. The reduction of bioactive proteins is probably related to the ice-induced denaturation. In conclusion, fast cooling and ultra-low constant temperature are more suitable for the cryopreservation of human milk.