Analysis of recombinants in female mouse meiosis.
Analysis of recombinants in female mouse meiosis.
复制标题
雌性小鼠减数分裂中的重组体分析。
DOI：
10.1007/978-1-62703-191-2_2
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Keeney,Scott
中科院分区：
文献类型：
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作者：
deBoer,Esther;Jasin,Maria;Keeney,Scott
在减数分裂过程中，同源染色体（同系物）经历重组相互作用，导致形成交换（CO）或非交换（NCO）。CO和NCO都是由相同的事件启动的：程序性双链DNA断裂（DSB），它优先发生在整个基因组的热点。CO有助于配子的遗传多样性，并需要促进适当的减数分裂染色体分离。因此，它们的形成受到严格控制。在小鼠中，优选的CO形成的位点在男性和女性染色体之间不同，在区域水平和个体热点水平上都是如此。使用（单侧）等位基因特异性PCR进行精子分型已被证明是一种强大的技术，可用于表征男性人类和小鼠染色体热点处的CO和所有可检测到的NCO。相比之下，很少有人知道女性减数分裂热点的属性。本章介绍了一种适应精子分型分析重组在热点，使用DNA分离的卵巢细胞悬浮液富含卵母细胞。
During meiosis, homologous chromosomes (homologs) undergo recombinational interactions, resulting in the formation of crossovers (COs) or noncrossovers (NCOs). Both COs and NCOs are initiated by the same event: programmed double-strand DNA breaks (DSBs), which occur preferentially at hotspots throughout the genome. COs contribute to the genetic diversity of gametes and are needed to promote proper meiotic chromosome segregation. Accordingly, their formation is tightly controlled. In the mouse, the sites of preferred CO formation differ between male and female chromosomes, both on a regional level and on the level of individual hotspots. Sperm typing using (half-sided) allele-specific PCR has proven a powerful technique to characterize COs and all detectable NCOs at hotspots on male human and mouse chromosomes. In contrast, very little is known about the properties of hotspots in female meiosis. This chapter describes an adaptation of sperm typing to analyze recombinants in a hotspot, using DNA isolated from an ovary cell suspension enriched for oocytes.