Peptide imprinted receptors for the determination of the small cell lung cancer associated biomarker progastrin releasing peptide
Peptide imprinted receptors for the determination of the small cell lung cancer associated biomarker progastrin releasing peptide
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DOI：
10.1016/j.chroma.2014.10.023
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发表时间：
2014-11-28
影响因子：
4.1
通讯作者：
Sellergren, Boerje
中科院分区：
文献类型：
--
作者：
Qader, Abed Abdel;Urraca, Javier;Sellergren, Boerje
肽印迹聚合物被开发用于检测前胃泌素释放肽（ProGRP）;低丰度的基于血液的小细胞肺癌生物标志物。聚合物靶向蛋白型九肽序列NLLGLIEAK，并用于在基于LCMS的定量之前选择性富集蛋白型肽。首先从96个聚合物的组合库中鉴定出具有最佳亲和特性的肽印迹聚合物。考察了功能单体、交联剂、致孔剂和模板剂对NLLGLIEAK吸附容量和选择性的影响。最后，建立了固相萃取法从胰蛋白酶解产物中高选择性富集目标肽的方法。(C)© 2014 Elsevier B. V.保留所有权利。
Peptide imprinted polymers were developed for detection of progastrin releasing peptide (ProGRP); a low abundant blood based biomarker for small cell lung cancer. The polymers targeted the proteotypic nona-peptide sequence NLLGLIEAK and were used for selective enrichment of the proteotypic peptide prior to LCMS based quantification. Peptide imprinted polymers with the best affinity characteristics were first identified from a 96-polymer combinatorial library. The effects of functional monomers, crosslinker, porogen, and template on adsorption capacity and selectivity for NLLGLIEAK were investigated and optimized. Ultimately, a solid phase extraction method was developed for highly selective enrichment of the target peptide from tryptic digests. (C) 2014 Elsevier B.V. All rights reserved.