Effects of (-)-epicatechin on a diet-induced rat model of cardiometabolic risk factors
Effects of (-)-epicatechin on a diet-induced rat model of cardiometabolic risk factors
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DOI：
10.1016/j.ejphar.2014.01.053
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发表时间：
2014-04-05
影响因子：
5
通讯作者：
Ramirez-Sanchez, Israel
中科院分区：
文献类型：
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作者：
Gutierrez-Salmean, Gabriel;Ortiz-Vilchis, Pilar;Ramirez-Sanchez, Israel
超重和肥胖与心脏代谢风险增加有关。治疗包括生活方式的改变和/或药物。然而，这种干预措施往往受到依从性差和/或严重副作用的限制。食用某些饮食产品，如可可，对心脏代谢风险因素产生积极影响。（-）-表儿茶素（EPI）是可可中最丰富的类黄酮，据报道可复制这种效果。然而，其作用机制尚未完全阐明。在高脂饮食诱导的肥胖及其相关的心脏代谢危险因素的大鼠模型中，我们通过管饲法给予1 mg/kg EPI，持续2周。终点包括体重增加、血压、血压升高和收缩压。我们还评估了食物摄入和粪便排泄。用Westerns方法检测线粒体功能和结构相关蛋白的表达。高脂饮食5周后，大鼠出现肥胖、高血糖、高血脂和收缩期高血压。EN能显著降低体重增加率、腹泻率和高血脂率。能量摄入和排泄之间的比例没有显着改变治疗。EPI恢复了肥胖诱导的骨骼肌和腹部组织sirtuins（SIRTs）、过氧化物酶体增殖物激活受体辅激活因子（PGC-1 α）、丝裂素、线粒体转录因子A（TFAM）、解偶联蛋白1（UCP 1）和脱碘酶水平的下降。EPI治疗对高脂饮食诱导的终点产生有益作用，因此可被视为治疗肥胖及其心脏代谢相关异常的潜在药物。作用机制可能归因于调节细胞/线粒体功能，从而改善整体代谢。(C)2014爱思唯尔有限公司版权所有。
Overweight and obesity have been associated with increase in cardiometabolic risk. Therapeutics include lifestyle changes and/or pharmacologic agents. However, such interventions are often limited by poor compliance and/or significant side effects. The consumption of certain dietary products, such as cocoa, exerts positive effects on cardiometabolic risk factors. (-)-Epicatechin (EPI), the most abundant flavonoid in cacao has been reported to replicate such effects. However its mechanisms of action have not been fully elucidated. In a rat model of high-fat diet-induced obesity and its associated crdiometabolic risk factors, we administered 1 mg/kg of EPI, by gavage, for 2 weeks. Endpoints included weight-gain, glycemia, triglyceridemia, and systolic blood pressure. We also assessed food intake and fecal excretion. Mitochondrial function and structure related proteins were measured by Westerns Obesity, hyperglycemia, hypertriglyceridemia, and systolic hypertension were developed after the administration of the high-fat diet for five weeks. EN significantly decreased the rate of weight gain, glycemia and hypertriglyceridemia. The ratio between energy intake and excretion was not significantly modified by treatment. EPI restored the obesity-induced decreases in the levels of skeletal muscle and abdominal tissue sirtuins (SIRTs), peroxisome proliferator-activated receptor coactivator (PGC-1 alpha), mitofilin, transcription factor A mitochondrial (TFAM), uncoupling protein 1 (UCP1), and deiodinase. EPI treatment yielded beneficial effects on high fat diet-induced endpoints thus may be considered as a potential agent for the treatment of obesity and its cardiometabolic associated abnormalities. Mechanism of action may be attributed to the modulation of cellular/mitochondrial function, thus improving overall metabolism. (C) 2014 Elsevier B.V. All rights reserved.