Thermogenesis activated by central melanocortin signaling is dependent on neurons in the rostral raphe pallidus (rRPa) area
Thermogenesis activated by central melanocortin signaling is dependent on neurons in the rostral raphe pallidus (rRPa) area
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DOI：
10.1016/j.brainres.2007.04.006
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发表时间：
2007-11-07
期刊：
影响因子：
2.9
通讯作者：
Yu, Pinxuan
中科院分区：
文献类型：
--
作者：
Fan, Wei;Morrison, Shaun F.;Yu, Pinxuan
中枢黑皮质素系统在调节能量平衡中起着关键作用，包括棕色脂肪组织（BAT）中的产热。下丘脑黑皮质素信号传导的激活刺激交感神经介导的肩胛间BAT（IBAT）产热。中缝苍白球吻端（rRPa）及其邻近区域被认为是中枢神经系统（CNS）控制IBAT产热的交感运动前神经元的位置。为了确定rRPa区神经元的活动是否是中枢黑皮质素诱导的产热所必需的，我们研究了抑制rRPa区神经元的活动对侧脑室注射黑皮质素3/4受体（MC 3/4 R）激动剂MTII引起的IBAT的交感神经活动（SNA）的影响，在清醒的自由活动的小鼠中，MTII对第三或第四脑室注射诱导的O-2消耗的影响。ICV注射MTII（1 nmol）显著增加大鼠IBAT SNA（+741%的对照）。第三和第四脑室注射MTII（1 nmol）显着增加清醒的C57 BL/6 J小鼠的O-2消耗（第三脑室注射比生理盐水对照高45%，第四脑室注射比生理盐水对照高44%）。MAT SNA和O-2消耗的增加被逆转的抑制神经元在rRPa和相邻区域的微量注射甘氨酸或蝇蕈醇到rRPa。这些结果表明，在RPa及其附近的神经元在介导的MAT产热的激活中枢黑皮质素信号诱导的增加中起着至关重要的作用。(c)2007年由Elsevier B. V.出版。
The central melanocortin system plays a critical role in regulation of energy balance, including thermogenesis in brown adipose tissue (BAT). Activation of the hypothalamic melanocortin signaling stimulates sympathetically-mediated interscapular BAT (IBAT) thermogenesis. The rostral raphe pallidus (rRPa) and adjacent area have been proposed as the location of sympathetic premotor neurons for the central nervous system (CNS) control of IBAT thermogenesis. To determine if neuronal activity in rRPa area is required for the central melanocortin-induced thermogenesis, we studied the effects of inhibition of the activity of neurons in the rRPa area on the sympathetic nerve activity (SNA) to IBAT evoked by lateral ventricular injection of the melanocortin 3/4 receptor (MC3/4R) agonist, MTII, in urethane-chloralose -anesthetized rats and the effects on O-2 consumption induced by third or fourth ventricular injection of MTII in conscious freely moving mice. Icv injection of MTII (1 nmol) significantly increased rat IBAT SNA (+741% of control). Both third and fourth ventricular injections of MTII (1 nmol) significantly increased O-2 consumption in conscious C57BL/6J mice (45% higher than that of saline control for third ventricular injection and 44% higher than that of saline control for fourth ventricular injection). The increases in MAT SNA and in O-2 consumption were reversed by inhibition of neurons in the rRPa and adjacent area with microinjections of glycine or muscimol into rRPa. These results suggest that the neurons in the RPa and its immediate vicinity play an essential role in mediating the increase in MAT thermogenesis induced by activation of central melanocortin signaling. (c) 2007 Published by Elsevier B.V.