Short Report: Performance of a Histidine-Rich Protein 2 Rapid Diagnostic Test, Paracheck Pf®, for Detection of Malaria Infections in Ugandan Pregnant Women
Short Report: Performance of a Histidine-Rich Protein 2 Rapid Diagnostic Test, Paracheck Pf®, for Detection of Malaria Infections in Ugandan Pregnant Women
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DOI：
10.4269/ajtmh.2012.10-0631
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发表时间：
2012-01-01
影响因子：
3.3
通讯作者：
Snounou, Georges
中科院分区：
文献类型：
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作者：
Dhorda, Mehul;Piola, Patrice;Snounou, Georges
改进实验室诊断对于减轻妊娠期疟疾负担至关重要 在分娩时进行的研究中，外周血涂片似乎不如基于富含组氨酸蛋白 2 的恶性疟原虫的胎盘疟疾感染快速诊断检测 (RDT) 敏感。在这项研究中，对乌干达 81 名怀孕中期或晚期的妇女进行了随访直至分娩。每次访视时，通过血涂片检测外周血。 RDT 和巢式物种特异性聚合酶链反应 (PCR)。测试的敏感性和特异性是通过 PCR 计算的，PCR 检测出 22 例恶性疟原虫感染，作为金标准。血涂片的敏感性和特异性分别为 36.4%（95% 置信区间 [CI] = 18.0-59.2%）和 99.6%（95% CI = 97.7-100%）。 RDT 的相应值为 31.8% (95% CI = 14.7-54.9%) 和 100% (95% CI = 98.3-100%)。由于 RDT 相对易于使用，因此可以取代血涂片来诊断妊娠期疟疾。迫切需要对妊娠期疟疾进行现场敏感测试。
Improved laboratory diagnosis is critical to reduce the burden of malaria in pregnancy Peripheral blood smears appear less sensitive than Plasmodium falciparum histidine-rich protein 2 based rapid diagnostic tests (RDTs) for placental malaria infections in studies conducted at delivery. In this study, 81 women in Uganda in the second or third trimester of pregnancy were followed-up until delivery. At each visit, peripheral blood was tested by blood smear. RDT, and nested species-specific polymerase chain reaction (PCR). Sensitivity and specificity of the tests was calculated with PCR, which detected 22 infections of P falciparum, as the gold standard. The sensitivity and specificity of blood smears were 36.4% (95% confidence interval [CI] = 18.0-59.2%) and 99.6% (95% CI = 97.7-100%), respectively. The corresponding values for RDT were 31.8% (95% CI = 14.7-54.9%) and 100% (95% CI = 98.3-100%). The RDTs could replace blood smears for diagnosis of malaria in pregnancy by virtue of their relative ease of use. Field-based sensitive tests for malaria in pregnancy are urgently needed.