Pseudomonas mosselii sp nov., a novel species isolated from clinical specimens
Pseudomonas mosselii sp nov., a novel species isolated from clinical specimens
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DOI：
10.1099/00207713-52-2-363
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发表时间：
2002-03-01
影响因子：
2.8
通讯作者：
Izard, D
中科院分区：
文献类型：
--
作者：
Dabboussi, F;Hamze, M;Izard, D
对22株临床来源的荧光假单胞菌（荧光假单胞菌10株、恶臭假单胞菌10株、假单胞菌2株）和33株假单胞菌属模式菌株的280个表型性状进行了数值分析。22个临床分离株中有12个聚集在一个特定的组（簇IV）内。其他菌株聚集在组内含有充分表征的荧光假单胞菌属物种或没有集群。属于簇IV的菌株在表型上不同于所有其他簇和亚簇的荧光假单胞菌。DNA-DNA杂交表明，簇IV对应于共享72-100%的DNA相关性的基因组。与狭义假单胞菌属30个种的67个菌株（包括最近描述的6个种（Veronii假单胞菌、罗得西亚假单胞菌、黎巴嫩假单胞菌、东方假单胞菌、雪松假单胞菌和Monteilii假单胞菌）的DNA-DNA杂交值低于49%。模式菌株的DNA G+C含量为63mol%。将簇IV的代表性菌株（CFML 90-83（T））的16 S rRNA基因序列与假单胞菌属的其它菌株的序列进行比较，揭示菌株CFML 90-83（T）是荧光曲霉属内簇的一部分。根据表型、DNA-DNA杂交和系统发育分析，提出的12个菌株的集群IV。模式菌株为莫氏毕赤酵母CFML 90-83（T）（= ATCC BAA-99（T）= CIP 105259（T））。莫氏毕赤酵母菌株在表型上是同质的，并且可以通过几种表型特征（包括绿脓菌荧光素分型）与其他荧光物种区分。
Twenty-two fluorescent pseudomonad strains of clinical origin received as Pseudomonas fluorescens (10 strains), Pseudomonas putida (10 strains) and Pseudomonas sp. (2 strains), and 33 type strains of the genus Pseudomonas were studied by numerical analysis based on 280 phenotypic characters. Twelve of the 22 clinical isolates clustered within a specific group, cluster IV. The other strains clustered within groups containing well-characterized fluorescent Pseudomonas species or did not cluster. Strains belonging to cluster IV were phenotypically different from all other clusters and subclusters of fluorescent pseudomonads. DNA-DNA hybridization showed that cluster IV corresponded to a genomic group sharing 72-100% DNA relatedness. DNA-DNA hybridization values with 67 strains representing 30 species of the genus Pseudomonas sensu stricto, including six recently described species (Pseudomonas veronii, Pseudomonas rhodesiae, Pseudomonas libanensis, 'Pseudomonas orientalis', 'Pseudomonas cedrella' and Pseudomonas monteilii, were below 49%, the value found for A monteilii. The DNA G+C content of the type strain was 63 mol%. Comparison of the 16S rRNA gene sequence of a representative strain of cluster IV (CFML 90-83(T)) with sequences of other strains of the genus Pseudomonas revealed that strain CFML 90-83(T) was part of the A fluorescens intrageneric cluster. On the basis of phenotypic, DNA-DNA hybridization and phylogenetic analyses, a novel species, Pseudomonas mosselii sp. nov., is proposed for the 12 strains of cluster IV. The type strain is P. mosselii CFML 90-83(T) (= ATCC BAA-99(T) = CIP 105259(T)). The P. mosselii strains are phenotypically homogeneous and can be differentiated from other fluorescent species by several phenotypic features, including pyoverdine typing.