Decreased expression of SPRR3 in Chinese human oesophageal cancer
Decreased expression of SPRR3 in Chinese human oesophageal cancer
复制标题
DOI：
10.1093/carcin/21.12.2147
复制
发表时间：
2000-12-01
期刊：
影响因子：
4.7
通讯作者：
Wu, M
中科院分区：
文献类型：
--
作者：
Chen, BS;Wang, MR;Wu, M
采用改进的mRNA差异显示技术分离了人食管癌与匹配的正常邻近粘膜之间差异表达的cDNA片段。其中一个被鉴定为SPRR3的3'-非翻译区，与食管cDNA同源，Northern blot、dot blot和逆转录聚合酶链反应（RT-PCR）分析显示，SPRR3在食管癌的3个细胞系中表达缺失，57例原发性食管癌中有54例与邻近正常粘膜相比表达显著降低。这些数据提示，食管可能是SPRR3基因的蛋白产物，SPRR3/食管mRNA表达改变是中国食管癌发展过程中的常见事件。
cDNA fragments that were differentially expressed between human oesophageal carcinomas and matched normal adjacent mucosa were isolated using an improved mRNA differential display technique. One of them was identified as the 3'-untranslated region of SPRR3 and was homologous to the esophagin cDNA, Northern blot, dot blot and reverse transcription-polymerase chain reaction (RT-PCR) analyses revealed that SPRR3 expression was lost in three cell lines of oesophageal carcinoma and was dramatically decreased in 54 out of 57 primary oesophageal carcinomas compared with adjacent normal mucosa, Esophagin has been shown to be down-regulated in western oesophageal carcinomas. The data suggest that esophagin is probably the protein product of the gene SPRR3 and that altered mRNA expression of SPRR3/esophagin is a frequent event in the development of Chinese oesophageal cancer.