Self-Mineralized Photothermal Bacteria Hybridizing with Mitochondria-Targeted Metal-Organic Frameworks for Augmenting Photothermal Tumor Therapy
Self-Mineralized Photothermal Bacteria Hybridizing with Mitochondria-Targeted Metal-Organic Frameworks for Augmenting Photothermal Tumor Therapy
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DOI：
10.1002/adfm.201909806
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发表时间：
2020-02-16
影响因子：
19
通讯作者：
Zhang, Xian-Zheng
中科院分区：
文献类型：
--
作者：
Chen, Qi-Wen;Liu, Xin-Hua;Zhang, Xian-Zheng
利用兼性厌氧细菌Shewanella oneidensis MR-1（S. oneidensis MR-1）在其表面上生物矿化钯纳米颗粒（Pd NPs）而不影响细菌活性。结果表明，PTB在近红外（NIR）区域具有良好的上级光热性能，并具有较好的肿瘤靶向性。将包封光敏剂亚甲蓝（MB）的沸石咪唑框架-90（ZIF-90）在活性PTB表面上杂交以进一步增强PTT功效。MB包封的ZIF-90（ZIF-90/MB）可以在线粒体处选择性地释放MB，并通过在光照下产生单线态氧（O-1（2））引起线粒体功能障碍。线粒体功能障碍进一步导致三磷酸腺苷（ATP）合成抑制和热休克蛋白（HSPs）表达下调。本文展示的PTB@ZIF-90/MB基于PTB的治疗平台将在克服PTT中肿瘤靶向和肿瘤热耐受性的挑战方面具有巨大潜力。
A photothermal bacterium (PTB) is reported for tumor-targeted photothermal therapy (PTT) by using facultative anaerobic bacterium Shewanella oneidensis MR-1 (S. oneidensis MR-1) to biomineralize palladium nanoparticles (Pd NPs) on its surface without affecting bacterial activity. It is found that PTB possesses superior photothermal property in near infrared (NIR) regions, as well as preferential tumor-targeting capacity. Zeolitic imidazole frameworks-90 (ZIF-90) encapsulating photosensitizer methylene blue (MB) are hybridized on the surface of living PTB to further enhance PTT efficacy. MB-encapsulated ZIF-90 (ZIF-90/MB) can selectively release MB at mitochondria and cause mitochondrial dysfunction by producing singlet oxygen (O-1(2)) under light illumination. Mitochondrial dysfunction further contributes to adenosine triphosphate (ATP) synthesis inhibition and heat shock proteins (HSPs) down-regulated expression. The PTB-based therapeutic platform of PTB@ZIF-90/MB demonstrated here will find great potential to overcome the challenges of tumor targeting and tumor heat tolerance in PTT.