Dissecting the linkage between transcription factor self-assembly and site-specific DNA binding: the role of the analytical ultracentrifuge.
Dissecting the linkage between transcription factor self-assembly and site-specific DNA binding: the role of the analytical ultracentrifuge.
复制标题
剖析转录因子自组装和位点特异性 DNA 结合之间的联系：分析超速离心机的作用。
DOI：
10.1007/978-1-61779-334-9_10
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Bain,DavidL
中科院分区：
文献类型：
--
作者：
Moody,AmieD;Robblee,JamesP;Bain,DavidL
A long-standing goal of biomedical research has been to determine the quantitative mechanisms responsible for gene regulation and transcriptional activation. These events occur through numerous protein–protein and protein–DNA interactions, many of which are allosterically coupled. For systems where highly purified protein is available, analytical ultracentrifugation provides a means to study these linked reactions, allosteric or otherwise. Sedimentation velocity is an ultracentrifugation technique that provides rigorous insight into protein self-association, homogeneity, and gross structure. Because self-association is often in dynamic equilibrium with other reactions such as DNA binding, an explicit and independent analysis of each interaction is critical to revealing mechanism. This chapter details a protocol for using sedimentation velocity to dissect the linkage between transcription factor self-association and site-specific DNA binding.
