RsrII--a novel restriction endonuclease with a heptanucleotide recognition site.
RsrII--a novel restriction endonuclease with a heptanucleotide recognition site.
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RsrII——一种具有七核苷酸识别位点的新型限制性内切核酸酶。
DOI：
10.1093/nar/12.17.6701
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发表时间：
1984
影响因子：
14.9
通讯作者：
J. Saunders
中科院分区：
文献类型：
--
作者：
C. O'Connor;E. Metcalf;C. Wrighton;T. Harris;J. Saunders
一个序列特异性核酸内切酶的提取物中存在的红球藻sphaeroides 630已被纯化和表征。酶RsrII识别并切割回文七核苷酸序列：（序列;参见测试）凭借其不寻常的特异性，RsrII很少切割大多数DNA分子，这应该有助于大基因组的物理作图。
A sequence-specific endonuclease present in extracts of Rhodopseudomonas sphaeroides 630 has been purified and characterized. The enzyme, Rsr II, recognises and cleaves the palindromic heptanucleotide sequence: (sequence; see test) By virtue of its unusual specificity, RsrII cuts most DNA molecules very infrequently which should facilitate the physical mapping of large genomes.