Differential regulation of nuclear receptors, neuropeptides and peptide hormones in the hypothalamus and pituitary of food restricted rats
Differential regulation of nuclear receptors, neuropeptides and peptide hormones in the hypothalamus and pituitary of food restricted rats
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DOI：
10.1016/j.molbrainres.2004.09.025
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发表时间：
2005-01-05
期刊：
影响因子：
--
通讯作者：
Schiöth, HB
中科院分区：
文献类型：
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作者：
Lindblom, J;Haitina, T;Schiöth, HB
食物限制与许多内分泌紊乱有关。我们验证了几个管家基因的实验条件，并使用实时定量聚合酶链反应 (PCR) 确定了 12 天 50% 食物限制对不同神经内分泌系统相关基因的下丘脑和垂体转录的影响。在大鼠下丘脑背侧和腹侧以及垂体中总共研究了 7 种核受体和 12 种神经肽和肽激素。在下丘脑中，食物限制降低了雌激素受体 a (ERα)、孕激素受体、糖皮质激素受体、甲状腺激素受体 α 和 β、阿片黑皮素原 (POMC)、生长激素释放因子 (GHRF)、促肾上腺皮质激素释放因子 (CRF)、促甲状腺激素释放因子 (TPF)、生长抑素的 mRNA 水平，并增加了神经肽 Y 的 mRNA 水平（NPY）。在垂体中，治疗减少了生长激素 (GH)、促黄体生成素 β (LHβ) 和促甲状腺素 β，但增加了 ERα mRNA 水平。该研究提供了一幅地图，显示食物限制如何影响神经内分泌控制中涉及的许多转录本的调节。 (C) 2004 Elsevier B.V. 保留所有权利。
Food restriction is associated with a number of endocrine disturbances. We validated the experimental conditions for several housekeeping genes and determined the effects of 12 day 50% food restriction on hypothalamic and pituitary transcription of genes involved in different neuroendocrine systems, using real-time quantitative polymerase chain reaction (PCR). A total of 7 nuclear receptors and 12 neuropeptides and peptide hormones were investigated in the dorsal and ventral hypothalamus and the pituitary gland in rats. In the hypothalamus, food restriction reduced mRNA levels of estrogen receptor a (ERalpha), progesterone receptor, glucocorticoid receptor, thyroid hormone receptor alpha and beta, pro-opiomelanocortin (POMC), growth hormone-releasing factor (GHRF), corticotropin-releasing factor (CRF), thyrotropin-releasing factor (TPF), somatostatin, and increased that of neuropeptide Y (NPY). In the pituitary, the treatment reduced growth hormone (GH), luteinizing hormone beta (LHbeta) and thyrotropin beta, but increased ERalpha mRNA levels. The study provides a map of how food restriction affects the regulation of a number of transcripts involved in neuroendocrine control. (C) 2004 Elsevier B.V. All rights reserved.